LOCUS       sequence-131283-        8287 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the extracellular region of the Neuropilin 1 protein, 
            C-terminally fused to the Fc region of human IgG1 + 6X histidine 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8287)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 8287)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Nrp1-Fc-His
FEATURES             Location/Qualifiers
     source          1..8287
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     regulatory      1606..1615
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4867..4884
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4910..5031
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4947..4966)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5001..5020
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5069..5086)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5240..5828)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5320..5339)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5999..6790,6791..6859))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6622..6641
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6860..6964)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7032..7050
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7088..7110)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7112..7132)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7117..7447
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7298..7433
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7360..7379
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     7584..7603
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(7670..8125)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7807..7828)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8019..8038
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8251..8273
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8265..8282
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8266..8282
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc caaacgccac catggagagg
     1621 gggctgccgt tgctgtgcgc cacgctcgcc cttgccctcg ccctggcggg cgctttccgc
     1681 agcgacaaat gtggcgggac cataaaaatc gaaaacccag ggtacctcac atctcccggt
     1741 taccctcatt cttaccatcc aagtgagaag tgtgaatggc taatccaagc tccggaaccc
     1801 taccagagaa tcatgatcaa cttcaaccca catttcgatt tggaggacag agactgcaag
     1861 tatgactacg tggaagtaat cgatggggag aatgaaggcg gccgcctgtg ggggaagttc
     1921 tgtgggaaga ttgcaccttc tcctgtggtg tcttcagggc cctttctctt catcaaattt
     1981 gtctctgact atgagacaca tggggcaggg ttttccatcc gctatgaaat cttcaagaga
     2041 gggcccgaat gttctcagaa ctatacagca cctactggag tgataaagtc ccctgggttc
     2101 cctgaaaaat accccaacag cttggagtgc acctacatca tctttgcacc aaagatgtct
     2161 gagataatcc tggagtttga aagttttgac ctggagcaag actcgaatcc tcccggagga
     2221 atgttctgtc gctatgaccg gctggagatc tgggatggat tccctgaagt tggccctcac
     2281 attgggcgtt attgtgggca gaaaactcct ggccggatcc gctcctcttc aggcgttcta
     2341 tccatggtct tttacactga cagcgcaata gcaaaagaag gtttctcagc caactacagt
     2401 gtgctacaga gcagcatctc tgaagatttt aagtgtatgg aggctctggg catggaatct
     2461 ggagagatcc attctgatca gatcactgca tcttcacagt atggtaccaa ctggtctgta
     2521 gagcgctccc gcctgaacta ccctgaaaat gggtggactc caggagaaga ctcctacaag
     2581 gagtggatcc aggtggactt gggcctcctg cgattcgtta ctgctgtagg gacacagggt
     2641 gccatttcca aggaaaccaa gaagaaatat tatgtcaaga cttacagagt agacatcagc
     2701 tccaacggag aggactggat ctccctgaaa gagggaaata aagccattat ctttcaggga
     2761 aacaccaacc ccacagatgt tgtcttagga gttttctcca aaccactgat aactcgattt
     2821 gtccgaatca aacctgtatc ctgggaaact ggtatatcta tgagatttga agtttatggc
     2881 tgcaagataa cagattatcc ttgctctgga atgttgggca tggtgtctgg acttatttca
     2941 gactcccaga ttacagcatc caatcaagcc gacaggaatt ggatgccaga aaacatccgt
     3001 ctggtgacca gtcgtaccgg ctgggcactg ccaccctcac cccacccata caccaatgaa
     3061 tggctccaag tggacctggg agatgagaag atagtaagag gtgtcatcat tcagggtggg
     3121 aagcaccgag aaaacaaggt gttcatgagg aagttcaaga tcgcctatag taacaatggc
     3181 tctgactgga aaactatcat ggatgacagc aagcgcaagg ctaagtcgtt cgaaggcaac
     3241 aacaactatg acacacctga gcttcggacg ttttcacctc tctccacaag gttcatcagg
     3301 atctaccctg agagagccac acacagtggg cttgggctga ggatggagct actgggctgt
     3361 gaagtggaag cacctacagc tggaccaacc acacccaatg ggaacccagt ggatgagtgt
     3421 gacgacgacc aggccaactg ccacagtggc acaggtgatg acttccagct cacaggaggc
     3481 accactgtcc tggccacaga gaagccaacc attatagaca gcaccatcca atcagagttc
     3541 ccgacatacg gttttaactg cgagtttggc tggggctctc acaagacatt ctgccactgg
     3601 gagcatgaca gccatgcaca gctcaggtgg agtgtgctga ccagcaagac agggccgatt
     3661 caggaccata caggagatgg caacttcatc tattcccaag ctgatgaaaa tcagaaaggc
     3721 aaagtagccc gcctggtgag ccctgtggtc tattcccaga gctctgccca ctgtatgacc
     3781 ttctggtatc acatgtccgg ctctcatgtg ggtacactga gggtcaaact acgctaccag
     3841 aagccagagg aatatgatca actggtctgg atggtggttg ggcaccaagg agaccactgg
     3901 aaagaaggac gtgtcttgct gcacaaatct ctgaaactat atcaggttat ttttgaaggt
     3961 gaaatcggaa aaggaaacct tggtggaatt gctgtggatg atatcagtat taacaaccat
     4021 atttctcagg aagactgtgc aaaaccaaca gacctagata aaaagaacac agaaattaaa
     4081 attgatgaaa cagggagcac tccaggatat gaaggagaag gggaaggtga caagaacatc
     4141 tccaggaagc caggcaatgt gcttaagacc ctgactagtg acaaaactca cacatgccca
     4201 ccgtgcccag cacctgaact cctgggggga ccgtcagtct tcctcttccc cccaaaaccc
     4261 aaggacaccc tcatgatctc ccggacccct gaggtcacat gcgtggtggt ggacgtgagc
     4321 cacgaagacc ctgaggtcaa gttcaactgg tacgtggacg gcgtggaggt gcataatgcc
     4381 aagacaaagc cgcgggagga gcagtacaac agcacgtacc gtgtggtcag cgtcctcacc
     4441 gtcctgcacc aggactggct gaatggcaag gagtacaagt gcaaggtctc caacaaagcc
     4501 ctcccagccc ccatcgagaa aaccatctcc aaagccaaag ggcagccccg agaaccacag
     4561 gtgtataccc tgcccccatc ccgggatgag ctgaccaaga accaggtcag cctgacctgc
     4621 ctggtcaaag gcttctatcc cagcgacatc gccgtggagt gggagagcaa tgggcagccg
     4681 gagaacaact acaagaccac gcctcccgtg ctggactccg acggctcctt cttcctctac
     4741 agcaagctca ccgtggacaa gagcaggtgg cagcagggga acgtcttctc atgctccgtg
     4801 atgcatgagg ctctgcacaa ccactacacg cagaagagcc tctccctgtc tccgggtaaa
     4861 ggcggccatc atcaccatca ccattaatct agagatctta ttaaagcaga acttgtttat
     4921 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     4981 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     5041 gtcgactcta gctagactct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     5101 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     5161 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     5221 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     5281 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     5341 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     5401 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     5461 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     5521 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     5581 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     5641 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     5701 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     5761 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     5821 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     5881 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     5941 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     6001 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     6061 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     6121 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     6181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     6241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     6301 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     6361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     6421 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     6481 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     6541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     6601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     6661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     6721 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     6781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     6841 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     6901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     6961 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     7021 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg
     7081 gtgaaaacct ctgacacatg cagctcccgg aggaatgtgt gtcagttagg gtgtggaaag
     7141 tccccaggct ccccaggcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     7201 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     7261 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     7321 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     7381 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     7441 ttgcaaaaag ctcccgggag cttgtatatc cattttcgtc ccggagacgg tcacagcttg
     7501 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     7561 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
     7621 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcagga aattgtaagc
     7681 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     7741 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
     7801 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     7861 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
     7921 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     7981 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     8041 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     8101 cttaatgcgc cgctacaggg cgcgtccatt cgccattcag gctgcgcaac tgttgggaag
     8161 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     8221 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     8281 gtgaatt
//
