LOCUS       sequence-13138-e        4713 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4713)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 4713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1768-HQ858782
FEATURES             Location/Qualifiers
     source          1..4713
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             112..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    1036..1047
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     protein_bind    complement(2270..2369)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2386..2405)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2387..2405)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2410..2426)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2410..2426)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2528..2547)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2539..3348
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2606..2625)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3495..4083
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3984..4003
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4237..4254
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4413..4440)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4532..4618)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4667..4689
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4681..4698
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4682..4698
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac
      121 tttaagaagg agcccttcac catgatgcac atgctggtag ccccggatag gggcggaggg
      181 gagctgcagc cgtacgtagc gccgcccgca gagcaggagc tggagctcct ccgcgacaac
      241 gccgatgacg gcctcgaggg ccacgtgcga tgcctaaggt gtggaatatc cggcaatgcg
      301 acgccccata tgcgccgggg gcctgatggc ccaagaactc tttgcaatgc gtgtggcatt
      361 gcttacagga aggggaaaat gagaagaatg atagaagctg aaccacccat agatgaggct
      421 gctcttgcaa agctggttcc agaggtaggc atggaatttg tgagtgagga aaaggcatac
      481 gaattctaca acaaatatgc tgggcatgtt ggctttagcg ttaggaaaag tacatcacat
      541 aaatcttcag aaaatattac caaagttaga acttttgtgt gctcaagaga aggctacaac
      601 agggataaaa aatcactgga agccaagaag ccaagattgg atacacgaat cggttgccca
      661 gcgagattga ttataaaagt cacaccagag tgcaagtata gagtaactga ttttaaagca
      721 gatcacaatc atcagctggc cccaccttca acgatgcata tgttaaggtc gcagaggata
      781 ttgacagaac ttcagcctgg agaagcagaa ttatcagatg attctgtagt gacaccaaca
      841 acaaaggcaa ctggtgatct tgttgtgaga caagtcagtt ttcttcggag tttatctctc
      901 ctcccagctg attacaagaa ttatctccgt tcgaagcgta tgaaggccat gcaacctggt
      961 gatggtggag caatattgaa atatttacag acaatgcaaa tggataaccc ctcgttcttt
     1021 tacactatgc agatcgatga ggatgacaaa ctgacaaact tcttttgggc cgaccaaaaa
     1081 tcgagagatg acttcaacta tttcggtgat gtgctctgct tagacacaac atacaaaata
     1141 aacggttatg gtagaccgct tgcgttattc cttggtgtga atcaccataa acaaacgatt
     1201 atttttggtg cagctatgct ttatgatgaa tcatttgatt catacaagtg gctttttgat
     1261 agttttaaga tcgccatgca tggaaaacag cctgcagtag ctttgataga tcaatctatt
     1321 ccattgagca gtgcaatggc agccgcctgg ccaagtacca ctcaacgaat ttgtgcttgg
     1381 catgtgtatc agaattctct taagcacctc aatcatgttt tccaaggttc gaagacattc
     1441 gcgaaggact tcggcaaatg tgtctttggc tacgaggatg aagatgaatt cgtgttttca
     1501 tggagaagta tgctggagaa atatgatctt agacataatg aatggttgtc taaagtgttt
     1561 gctgagaagg aacagtgggc cttggcatat gatagacata tattttgcgc tgacatcata
     1621 agtgcacttc aagccgagag tttcagtagt attttgaaga aattcttgag tcctcaactg
     1681 gagctgctgt ccttcttcaa gcactatgaa agagcagtgg acgaacatcg ctatgctgag
     1741 ctgcaagccg acttccaagc tagtcagagc tatccaagaa tacccccagc caagatgtta
     1801 aagcaaactg ctcatacata taccccggtg gtatttgaga tctttcgtaa agagtttgag
     1861 ctattcatgg actctgtgtt attcagttgt ggggaggctg ggacgacatc tgagtacaag
     1921 gttgcttcat ctgagaaacc caaggagcac tttgttcggt tcgattcaag tgattgctcc
     1981 tgtctgtgca cttgtaggaa gtttgagttt atgggtatcc cgtgctgtca catgttgaag
     2041 gtgctagatt acagaaatat caaagagctt cctcaaaaat atctgttgaa acggtggaga
     2101 aggaccgcca aatctgcaaa tgaagacaat gaaggcaatg caacaaacgc caacaacggg
     2161 tcattattga ttgtccctgc tcctcctaca aatcaccatg ggcttcagag cttcagtgca
     2221 atgattcaag atgcttcagt ttctagcatg cataagggtg ggcgcgccga cccagctttc
     2281 ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc
     2341 actatcagtc aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg
     2401 tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt
     2461 tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac
     2521 agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat
     2581 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca
     2641 ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat
     2701 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg
     2761 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta
     2821 ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca
     2881 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt
     2941 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg
     3001 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa
     3061 caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat
     3121 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat
     3181 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc
     3241 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct
     3301 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt
     3361 aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc aagctcatga
     3421 ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag
     3481 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     3541 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     3601 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     3661 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     3721 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     3781 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     3841 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagcattg agaaagcgcc
     3901 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     3961 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     4021 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     4081 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     4141 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     4201 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     4261 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     4321 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt
     4381 accgctagcc aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct
     4441 gcttagtttg atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt
     4501 gcttcacaac gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg
     4561 acaaacaaca gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat
     4621 gcctggcagt tccctactct cgcgttaacg ctagcatgga tgttttccca gtcacgacgt
     4681 tgtaaaacga cggccagtct taagctcggg ccc
//
