LOCUS       sequence-131399-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  inducible expression of TRP32 in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Luo T, Zhang X, Wakeel A, Popov VL, McBride JW
  TITLE     A variable-length PCR target protein of Ehrlichia chaffeensis 
            contains major species-specific antibody epitopes in acidic 
            serine-rich tandem repeats.
  JOURNAL   Infect Immun. 2008 Apr;76(4):1572-80. doi: 10.1128/IAI.01466-07. 
            Epub 2008 Jan 22.
  PUBMED    18212082
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/IAI.01466-07"; journalName: "Infect Immun"; date: 
            "2008-04"; volume: "76"; issue: "4"; pages: "1572-80"
COMMENT     Sequence Label: pBAD-TRP32
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             46..68
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             80..409
                     /codon_start=1
                     /product="His-Patch thioredoxin"
                     /label=HP-thioredoxin
                     /note="The histidines at positions 8, 32, and 64 coordinate
                     a divalent cation, allowing HP-thioredoxin to be purified 
                     on metal-chelating resins."
                     /translation="MGSDKIIHLTDDSFDTDVLKADGAILVDFWAHWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDHNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEF
                     LDANLA"
     CDS             425..439
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 ctctctactg tttctccata cccgtttttt tgggctagaa ataattttgt ttaactttaa
       61 gaaggagata tacataccca tgggatctga taaaattatt catctgactg atgattcttt
      121 tgatactgat gtacttaagg cagatggtgc aatcctggtt gatttctggg cacactggtg
      181 cggtccgtgc aaaatgatcg ctccgattct ggatgaaatc gctgacgaat atcagggcaa
      241 actgaccgtt gcaaaactga acatcgatca caacccgggc actgcgccga aatatggcat
      301 ccgtggtatc ccgactctgc tgctgttcaa aaacggtgaa gtggcggcaa ccaaagtggg
      361 tgcactgtct aaaggtcagt tgaaagagtt cctcgacgct aacctggccg gctctggatc
      421 cggtgatgac gatgacaagc tcgcccttat gtcacaattc tctgaagata atatgggtaa
      481 tatacaaatg ccttttgatt ctgattcaca tgagccttct catcttgagc tacctagtct
      541 ttctgaagaa gtgattcaat tagagagtga tctacaacaa tcttctaatt ctgatttaca
      601 cgggtctttt tctgttgagt tatttgatcc ttttaaagaa gcagttcaat tggggaatga
      661 tctacaacaa tcttctgatt ctgatttaca cgggtctttt tctgttgagt tatttgatcc
      721 ttctaaagaa gaagttcaat tggagagtga tctacaacaa tcttctaatt ctgatttaca
      781 cgagtcttct tttgttgagt tacctggtcc ttccaaagaa
//
