LOCUS       sequence-131400-         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  inducible expression of TRP32 in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Luo T, Zhang X, Wakeel A, Popov VL, McBride JW
  TITLE     A variable-length PCR target protein of Ehrlichia chaffeensis 
            contains major species-specific antibody epitopes in acidic 
            serine-rich tandem repeats.
  JOURNAL   Infect Immun. 2008 Apr;76(4):1572-80. doi: 10.1128/IAI.01466-07. 
            Epub 2008 Jan 22.
  PUBMED    18212082
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/IAI.01466-07"; journalName: "Infect Immun"; date: 
            "2008-04"; volume: "76"; issue: "4"; pages: "1572-80"
COMMENT     Sequence Label: pBAD-TRP32
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..46)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(56..97)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(716..730)
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 acagccagct ggagaccgtt taaactcaat ggtgatggtg atgatgaccg gtacgcgtag
       61 aatcgagacc gaggagaggg ttagggatag gcttaccttc aagctcgccc ttctctaaac
      121 tactttcact acagtgatga caataatctg gagtaacata atcaaaataa taataaggat
      181 tataacaaca ataatgatga taataatacg gtgtataacc agacaaataa ttcattgtac
      241 taaaaacacc acctaacaat aagcctaatt cagataaact aacatggtct tgtccatata
      301 ctacattttt agcatcatct tcgaattgaa cttcttcttt ggaaggacca ggtaactcaa
      361 caaaagaaga ctcgtgtaaa tcagaattag aagattgttg tagatcactc tccaattgaa
      421 cttcttcttt agaaggatca aataactcaa cagaaaaaga cccgtgtaaa tcagaatcag
      481 aagattgttg tagatcattc cccaattgaa ctgcttcttt aaaaggatca aataactcaa
      541 cagaaaaaga cccgtgtaaa tcagaattag aagattgttg tagatcactc tctaattgaa
      601 tcacttcttc agaaagacta ggtagctcaa gatgagaagg ctcatgtgaa tcagaatcaa
      661 aaggcatttg tatattaccc atattatctt cagagaattg tgacataagg gcgagcttgt
      721 catcgtcatc accggatcca gagccggcca ggttagcgtc gaggaactct ttcaactgac
      781 ctttagacag tgcacccact ttggttgccg ccacttcacc gtt
//
