LOCUS       sequence-131502-         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  An AAV packaging vector that expresses ER-retained low-affinity 
            GCaMP3 variant under control of the EF1a promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Henderson MJ, Baldwin HA, Werley CA, Boccardo S, Whitaker LR, Yan X,
            Holt GT, Schreiter ER, Looger LL, Cohen AE, Kim DS, Harvey BK
  TITLE     A Low Affinity GCaMP3 Variant (GCaMPer) for Imaging the Endoplasmic 
            Reticulum Calcium Store.
  JOURNAL   PLoS One. 2015 Oct 9;10(10):e0139273. doi: 
            10.1371/journal.pone.0139273. eCollection 2015.
  PUBMED    26451944
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-10-9"; volume: "10(10):e0139273. doi"; pages: " 
            10.1371/journal.pone.0139273. eCollection 2015"
COMMENT     Sequence Label: pOTTC810 - pAAV-EF1a-CRTsigpep-GCaMP3
            (D324G+D...
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(91..531)
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKGGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDAGGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKGGNGYISAAEL
                     RHVMTNLGEKLTDEEVDEMIREADIDGGGQVNYEEFVQMMTAK"
     primer_bind     647..666
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     906..927
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 atctttcaca aattttgtaa tccagaggtt gattatcgat aagcttgata tcgaattcgg
       61 cgcgccctac aactcatctt tttctgctgt cttcgctgtc atcatttgta caaactcttc
      121 gtagtttacc tgaccacccc catcgatgtc tgcttccctg atcatttcat caacctcttc
      181 atctgttaac ttctctccaa ggtttgtcat cacgtggcga agctctgctg cactgatgta
      241 gccattgcca cccttatcaa acacacggaa cgcttctcta atttcttctt cactgtctgt
      301 gtctttcatt tttcttgcca tcattgtcag gaactcaggg aagtcgattg tgccgtcacc
      361 gccggcatct acttcattga tcatgtcctg cagctctgct tctgtggggt tctgccccag
      421 agaccgcatc accgtcccca gctccttggt tgttattgtc ccatccccgc ccttgtcaaa
      481 tagggagaaa gcctctttaa attctgcgat ctgctcttca gtcagttggt cacgcgtgtt
      541 gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc
      601 gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc
      661 gtcgtccttg aagaagatgg tgcgctcctg gatgtagcct tcgggcatgg cggacttgaa
      721 gaagtcgtgc tgcttcatgt ggtcggggta gcggctgaag cactgcacgc cgtaggtcag
      781 ggtggtcacg agggtgggcc agggcacggg cagcttgccg gtggtgcaga tgaacttcag
      841 ggtcagcttg ccgtaggtgg catcgccctc accctcgccg gacacgctga acttgtggcc
      901 gtttacgtcg ccgtccagct cgaccaggat gggcaccacc ccggtgaaca gctcctcgcc
      961 cttgct
//
