LOCUS       sequence-131522-        9668 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A fusion of the Kif18b motor protein to the v1 SunTag peptide array.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9668)
  AUTHORS   Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD
  TITLE     A Protein-Tagging System for Signal Amplification in Gene Expression
            and Fluorescence Imaging.
  JOURNAL   Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 
            10.1016/j.cell.2014.09.039.
  PUBMED    25307933
REFERENCE   2  (bases 1 to 9668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039."
COMMENT     Sequence Label: pcDNA4TO-Kif18b-24xGCN4_v1
FEATURES             Location/Qualifiers
     source          1..9668
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     720..740
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        725..769
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     protein_bind    771..789
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    792..810
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     820..844
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3598..3663
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             3679..3744
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             3760..3825
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             3841..3906
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             3922..3987
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4003..4068
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4084..4149
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4165..4230
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4246..4311
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4327..4392
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4408..4473
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4489..4554
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     primer_bind     4570..4592
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     primer_bind     4593..4615
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             4618..4683
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4699..4764
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4780..4845
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4861..4926
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4942..5007
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             5023..5088
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             5104..5169
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             5185..5250
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             5266..5331
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             5347..5412
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             5428..5493
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             5509..5574
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     primer_bind     complement(5679..5696)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5685..5909
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5955..6383
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6042..6061)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6252..6273
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6392..6412)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6397..6726
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6577..6712
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6639..6658
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6774..6821
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6840..7214
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7344..7465
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7381..7400)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7435..7454
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7514..7530)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7514..7530)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7527..7549)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7538..7554
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7562..7568,7569..7586,7587..7592))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7607..7628
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7745..7762)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7916..8501)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7996..8015)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8672..9463,9464..9532))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9295..9314
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9533..9637)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcag ctcagctttc tagaccgcgc
      721 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc tccctatcag
      781 tgatagagat ctccctatca gtgatagaga tcgtcgacga gctcgtttag tgaaccgtca
      841 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc
      901 cagcctccgg actctagcgt ttaacttaag cttggtaccg agctcggatc caccgccatg
      961 gcagtggagg acagcacgct gcaagtagtg gtacgggtgc ggccccccac ccctcgggag
     1021 ctggacagtc agcggcggcc agtggttcag gtggtggacg agcgggtgct ggtgtttaac
     1081 cctgaggagc ccgatggagg gttccctggc ctgaaatggg gtggcaccca tgatggcccc
     1141 aagaagaagg gcaaagacct gacgtttgtc tttgaccggg tctttggcga ggcggccacc
     1201 caacaggacg tgttccagca caccacgcac agcgtcctgg acagcttcct ccagggctac
     1261 aactgctcag tgtttgccta cggggccacc ggggctggga agacacacac catgctggga
     1321 agggaggggg accccggcat catgtacctg accaccgtgg aactgtacag gcgcctggag
     1381 gcccgccagc aggagaagca cttcgaggtg ctcatcagct accaggaggt gtataatgaa
     1441 cagatccatg acctcctgga gcccaagggg ccccttgcca tccgcgagga ccccgacaag
     1501 ggggtggtgg tgcaaggact ttctttccac cagccagcct cagccgagca gctgctggag
     1561 atactgacca gggggaaccg taaccgcacg cagcacccca ctgatgccaa cgcgacttcc
     1621 tcccgctccc atgccatctt ccagatcttt gtgaagcagc aggaccgggt tccaggactg
     1681 acccaggctg tccaggtggc caagatgagc ctgattgacc tggctggctc agagcgggca
     1741 tccagcaccc atgcgaaggg ggagcggctg cgggaggggg ccaacatcaa ccgctctctg
     1801 ctggcgctca tcaacgtcct caatgccttg gccgatgcaa agggccgcaa gacccatgtg
     1861 ccctaccggg acagcaaact gacccgcctg ctcaaagact ccctcggggg caactgccgc
     1921 acagtgatga tcgctgccat cagcccctcc agcctgacct acgaggacac gtataatacc
     1981 ctcaaatatg ccgaccgggc caaggagatc aggctctcgc tgaagagcaa tgtgaccagc
     2041 ctggactgtc acatcagcca gtatgctacc atctgccaac agctccaggc tgaggtagcc
     2101 gctctgagga agaagctcca agtgtatgag gggggaggcc agcccccacc acaggacctc
     2161 ccaggatctc ccaagtcggg accaccacca gaacaccttc ccagctcccc cttgccaccc
     2221 caccctccca gccagccctg caccccagag ctccctgcag ggcctagagc ccttcaagag
     2281 gagagtctgg ggatggaggc ccaggtggag agggccatgg aagggaactc ttcagaccag
     2341 gagcagtccc cagaggatga ggatgaaggc ccagctgagg aggttccaac ccagatgcca
     2401 gagcagaacc ccacacatgc actgccagag tcccctcgcc tgaccctgca gcccaagcca
     2461 gtcgtgggcc acttctcagc acgggaactg gatggggacc gttctaagcg gttggcccta
     2521 aaggtgctgt gcgttgccca gcggcagtac tccctgctcc aagcagccaa cctcctgacg
     2581 cccgacatga tcacagagtt tgagacccta cagcagctgg tgcaagagga aaaaattgag
     2641 cctggggcag aggccttgag gacttcaggc ctggccaggg gggcacctct ggctcaggag
     2701 ctgtgttcag agtcaaagcc tccaggatac actggccctg tgacccggac tatggcgagg
     2761 cgactgagtg gccccctgca caccctggga atcccgcctg gacccaactg caccccagcc
     2821 caggggtccc gatggcccat ggagaagaag aggaggagac caagcgcctt ggaggcagac
     2881 agtcccatgg ccccaaagcg gggcaccaag cgccagcgcc agtccttcaa taactgccta
     2941 aggagagggt ctctgcctga cacccaacct tcacaggggc ccagcacccc caaaggagaa
     3001 agggcctcct ccccctgcca ttcccctcgc gtttgcccag ccacagtcat caaaagccgg
     3061 gtgcccctgg gcccttccgc catgcagaac tgctccaccc cgctggctct gcccactcga
     3121 gacctcaatg ccacctttga tctctctgag gagcctccct caaagcccag tttccatgaa
     3181 tgcattggct gggacaaaat accccaggag ctgagcaggc tggaccagcc cttcatcccc
     3241 agggcacctg tgcccctgtt caccatgaag ggccccaagc caacatcttc cctccctggg
     3301 acctctgcct gcaagaagaa gcgcgttgcg agttcctcag tctcccatgg ccgcagccgc
     3361 atcgcccgcc tccccagcag cactttgaag aggccagctg ggccccttgt actcccagag
     3421 ctgcccttga gtcccctgtg ccctagcaac cggaggaatg gaaaggacct catcagggtg
     3481 gggagagcac tctcagcagg gaacggcgtc accaaggtgt ccgcggccgc agatcgatcg
     3541 atcacgcgtg ccaccatgac tagttttgta caaagcggtc cgggaggttc tggaggattg
     3601 ttgcccaaaa actaccactt ggagaacgag gtcgcaaggt tgaaaaaatt ggtcggtgag
     3661 cgtggcggct caggcggcct tcttccaaaa aattatcatc ttgaaaatga agttgctaga
     3721 cttaaaaaac ttgttggaga aagaggcggg agcggcggcc tgctgcccaa gaactaccac
     3781 ctggagaacg aggtcgcccg cctgaagaag ctggtcggcg agcgcggtgg ttctggaggt
     3841 ttgctcccaa agaactacca cttggaaaac gaagtcgcaa gattgaagaa gttggtcggt
     3901 gaaagaggag gaagcggagg attattacct aaaaattatc atttggaaaa tgaagttgct
     3961 agattgaaga agttggttgg agagagaggg gggtctggag gactactacc gaaaaactac
     4021 cacctagaga acgaggtggc ccgactaaaa aagctagtgg gcgagcgcgg cggcagtggc
     4081 ggcctcctcc ccaagaatta ccacctcgaa aatgaagtag caaggctcaa gaagctcgta
     4141 ggagaaagag gtggttccgg gggactcctt ccaaaaaact atcatcttga aaacgaagtt
     4201 gcacgtctta aaaaacttgt tggcgaacgt ggcggctctg gcgggctgct gcccaagaat
     4261 taccacctgg aaaatgaagt ggcccgcctg aagaagctgg tgggcgaacg cggaggcagt
     4321 ggaggcctac taccgaagaa ctaccaccta gagaacgagg tggcacgcct aaagaagcta
     4381 gtgggcgagc gcggcggctc gggtggttta ttacctaaga attatcattt agaaaatgaa
     4441 gtagctcgat taaagaagtt agtaggtgaa cgtggtgggt ctggtggtct tcttccgaag
     4501 aactaccacc ttgagaacga ggtagctcgt cttaagaaac ttgtaggtga gcgtggtgga
     4561 agcggtggtg ggctggcaag ccacgtttgg tgccgggagc tgcatgtgtc agaggaactt
     4621 ttgccaaaga attatcatct tgagaacgaa gtggctcgtc ttaagaaact cgtaggagaa
     4681 agaggtggca gtggaggtct gcttcccaag aattaccacc tggaaaatga ggtagctaga
     4741 ctgaaaaagc tcgtaggcga gcgcggggga agtgggggat tgctgccaaa aaattatcat
     4801 ttggagaacg aagtagcacg actaaagaaa ttagttgggg agaggggggg atcgggtggc
     4861 ttactcccga aaaattatca tctcgaaaac gaagtggctc ggctaaaaaa gttagttgga
     4921 gagcgtggcg gttctggagg cctattacca aaaaactacc acctcgaaaa tgaggtggca
     4981 cgcttaaaaa agctggtcgg agaacgggga ggcagtggtg gcctactacc caagaattat
     5041 catcttgaga acgaggtagc gcgtttgaag aagctcgtcg gtgaaagagg tggctcagga
     5101 gggctgctcc caaagaacta tcatcttgaa aatgaggtcg ctcgattaaa aaagttagtg
     5161 ggcgagaggg gaggcagtgg tgggctactt cctaagaatt accacctcga aaacgaagta
     5221 gctcgattaa agaaattggt cggtgagaga ggtgggtcgg gtggcttact gccgaaaaat
     5281 tatcatctgg aaaatgaggt agcgagacta aaaaagctcg taggtgagag agggggttct
     5341 ggcggcttgc taccaaaaaa ttatcatctt gagaacgaag ttgctaggct caaaaagctg
     5401 gttggagagc gcggcggctc aggcggtctg ttacccaaaa actaccactt ggaaaatgag
     5461 gttgccagat tgaagaaact ggtcggcgag cgcggtggaa gtggaggact acttcctaaa
     5521 aattatcatt tggagaacga agtcgctaga ctgaaaaagt tggttgggga acgaggaggg
     5581 tctggcggca agctagcgat cggatcgatc gccgcggtaa acatatggat cgatcataat
     5641 actcgagtct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
     5701 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     5761 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     5821 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     5881 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     5941 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     6001 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     6061 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     6121 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     6181 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     6241 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     6301 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     6361 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
     6421 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     6481 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     6541 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     6601 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     6661 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     6721 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca
     6781 attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca
     6841 tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg
     6901 agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg
     6961 tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca
     7021 acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg
     7081 tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc
     7141 cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg
     7201 aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt
     7261 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     7321 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
     7381 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     7441 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
     7501 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     7561 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     7621 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     7681 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     7741 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     7801 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     7861 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     7921 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     7981 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     8041 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     8101 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     8161 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     8221 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     8281 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     8341 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     8401 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtttttttg
     8461 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     8521 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     8581 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     8641 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     8701 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     8761 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     8821 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     8881 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     8941 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     9001 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     9061 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     9121 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     9181 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     9241 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     9301 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     9361 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     9421 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     9481 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     9541 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     9601 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     9661 ctgacgtc
//
