LOCUS       sequence-13158-e        3801 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Yilmaz A, Nishiyama MY Jr, Fuentes BG, Souza GM, Janies D, Gray J, 
            Grotewold E
  TITLE     GRASSIUS: a platform for comparative regulatory genomics across the 
            grasses.
  JOURNAL   Plant Physiol. 2009 Jan;149(1):171-80. doi: 10.1104/pp.108.128579. 
            Epub 2008 Nov 5.
  PUBMED    18987217
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.108.128579"; journalName: "Plant Physiol"; date: 
            "2009-01"; volume: "149"; issue: "1"; pages: "171-80"
COMMENT     Sequence Label: pUT1180-HQ858802
FEATURES             Location/Qualifiers
     source          1..3801
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1358..1457)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1474..1493)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1475..1493)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1498..1514)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1498..1514)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1616..1635)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1627..2436
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1694..1713)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2583..3171
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3072..3091
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3325..3342
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3501..3528)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3620..3706)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3755..3777
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3769..3786
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3770..3786
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc
      121 atgggcgctc tttgtgattt ctgcggggag cagaggtcta tggtttattg ccgatcagat
      181 gcagcatcct tgtgcttatc gtgtgaccgt aatgtgcatt cagctaatgc tctttctcgg
      241 cgccatacaa ggacacttct gtgtgataga tgtgcttcac agcctgccat ggtccgctgc
      301 cttgtagaaa atgcctcgct ttgccaaaat tgtgattgga atgggcatag tgctgggtct
      361 tcagctgctg gtcataagag gcagaccata aactgctact cagggtgccc atcatcttca
      421 gaactttcaa aaatctggac atttgtttcg gatattccta atgtagctcc tgagccgaat
      481 tgcgagcaag ggataagcat gatgagcatc agcgacagtg gtgtcagtaa tcaagacaat
      541 gctgcagggg acagcagttt gttagatatt gctagtgcaa cccttatgag tgatcttggc
      601 actgctggca agccaaagtc tttgataggc tcctcatctg aagctggcgt gaaccttctt
      661 ccacttgcta ctgatcagat ggctggatcc gtggattcga catcagctaa ggtgccctat
      721 acggcagacc aagatatgtt cagcaaagac agtatatatg aagatttctg tgtggacgat
      781 gttgatctta gtttcgaaaa ttatgaggaa ctatttggca cctcccatat tcaaacagag
      841 caactgtttg atgatgctgg gatcgacagt tactttgaat cgaaggaaat accttctgga
      901 aactctgatg agcccaagct gatgcagcca gtgaccagca atgcagtatc tgctgattca
      961 gggatgtcga tcccaggagc caaaggtgat tccagtcttt gtatacctgt gaggcaagct
     1021 aggtccagta tctctctttc attttctggt ttgactggtg agagcagtgc tggagattac
     1081 caagattgtg gggtatcacc agtgctcctt atgggtgagc ctccttggca tcctcctggc
     1141 cctgaggggt catttgctgg agctaccaga gacgatgcta tcacacgata caaggagaag
     1201 aagaagagaa gaaaatttga caagaaaatc aggtatgcat ctcgcaaggc tagggcagat
     1261 gtgagaaaaa gagtcaaggg ccggtttgtt aaggctggtg aagcatacga ctacgatcca
     1321 ctctgtgaaa cgaggagcta caagggtggg cgcgccgacc cagctttctt gtacaaagtt
     1381 ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa
     1441 aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta ttacatggtc
     1501 atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa
     1561 gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
     1621 ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat
     1681 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc
     1741 tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc
     1801 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct
     1861 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg
     1921 atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt
     1981 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct
     2041 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg
     2101 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa
     2161 gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca
     2221 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc
     2281 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
     2341 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
     2401 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa
     2461 cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc aaaatccctt
     2521 aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     2581 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     2641 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     2701 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     2761 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     2821 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     2881 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     2941 acctacaccg aactgagata cctacagcgt gagcattgag aaagcgccac gcttcccgaa
     3001 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3061 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3121 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     3181 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     3241 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     3301 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     3361 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     3421 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac cgctagccag
     3481 gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttagtttgat
     3541 gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt
     3601 tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga
     3661 taaaacgaaa ggcccagtct tccgactgag cctttcgttt tatttgatgc ctggcagttc
     3721 cctactctcg cgttaacgct agcatggatg ttttcccagt cacgacgttg taaaacgacg
     3781 gccagtctta agctcgggcc c
//
