LOCUS       sequence-131772-         568 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 568)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB111
FEATURES             Location/Qualifiers
     source          1..568
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(96..220)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             231..296
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      315..567
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 ccaccgcgaa aatgacatca aaaacgccat taacctgatg ttctggggaa tataaatgtc
       61 aggctccctt atacacagcc agtctgcagg tcgaccatag tgactggata tgttgtgttt
      121 tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat
      181 cattttacgt ttctcgttca gctttcttgt acaaagtggt tgataacagc gactacaaag
      241 accatgacgg tgattataaa gatcatgaca tcgactacaa ggatgacgat gacaagtaga
      301 gctcgaattt ccccgatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg
      361 ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa
      421 ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat
      481 tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc
      541 gcgcggtgtc atctatgtta ctagatcg
//
