LOCUS       sequence-131777-         657 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 657)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB105
FEATURES             Location/Qualifiers
     source          1..657
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     184..205
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(271..616)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(308..328)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 agtcgtgctg cttcatgtgg tcggggtagc gggcgaagca catcaggccg tagccgaagg
       61 tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatg aacttcaggg
      121 tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt
      181 ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc tcctcgccct
      241 tgctcaccat gctcattcta gagtcccccg tgttctctcc aaatgaaatg aacttcctta
      301 tatagaggaa gggtcttgcg aaggatagtg ggattgtgcg tcatccctta cgtcagtgga
      361 gatatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt tttccacgat
      421 gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat cttcaacgat
      481 ggcctttcct ttatcgcaat gatggcattt gtaggagcca ccttcctttt ccactatctt
      541 cacaataaag tgacagatag ctgggcaatg gaatccgagg aggtttccgg atattaccct
      601 ttgttgaaaa gtctcaattg ccctttggtc ttctgagact gtatctttga tattttt
//
