LOCUS       sequence-13181-e        3213 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Yilmaz A, Nishiyama MY Jr, Fuentes BG, Souza GM, Janies D, Gray J, 
            Grotewold E
  TITLE     GRASSIUS: a platform for comparative regulatory genomics across the 
            grasses.
  JOURNAL   Plant Physiol. 2009 Jan;149(1):171-80. doi: 10.1104/pp.108.128579. 
            Epub 2008 Nov 5.
  PUBMED    18987217
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.108.128579"; journalName: "Plant Physiol"; date: 
            "2009-01"; volume: "149"; issue: "1"; pages: "171-80"
COMMENT     Sequence Label: pUT1323-HQ858825
FEATURES             Location/Qualifiers
     source          1..3213
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(770..869)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(886..905)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(887..905)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(910..926)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(910..926)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1028..1047)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1039..1848
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1106..1125)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1995..2583
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2484..2503
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2737..2754
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2913..2940)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3032..3118)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3167..3189
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3181..3198
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3182..3198
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc
      121 atgctgtccc acgtcgagat ggccccggcg gccggcgggt tcaagctgtt cggcaaggtc
      181 atcatgcagt gcggcgtttc cgaggggaca caggacaagg cgcagggctt cgtcgtggcc
      241 cgggagaagg ttgaaccgga ggaggaggag gaggaggagc agcgtgtccc ggctgctgct
      301 acgtcggggc agcgcgcgtc gatcaagcgg gaggccgcgg accgcgacga ggagcaacgg
      361 caagggggcg gggacgcggc ggggcagccg acgcagcggc ggttgcagga ctcggccgag
      421 gcgcgtgcgg ccgccgcggc gccgctgccg tgcccgcggt gccggagccg tgacaccaag
      481 ttctgctact tcaacaacta caacgtcaac cagccgcgcc acttctgcaa ggcctgccac
      541 cgctactgga cggccggcgg cgcgctccgc aacgtccccg tcggcgccgg ccgccgcaag
      601 aaccgtcccc tgggccccct cgcggtcgcc caccataacc accaccaccg cgcggccgcc
      661 ggcttcgtcc tcggcttccc caacccgtcc tccccaacct ccccgtcgcc ggtctacacc
      721 gaccggtggc cggtcacccc ggaccgcccg tttaagggtg ggcgcgccga cccagctttc
      781 ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc
      841 actatcagtc aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg
      901 tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt
      961 tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac
     1021 agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat
     1081 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca
     1141 ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat
     1201 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg
     1261 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta
     1321 ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca
     1381 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt
     1441 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg
     1501 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa
     1561 caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat
     1621 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat
     1681 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc
     1741 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct
     1801 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt
     1861 aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc aagctcatga
     1921 ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag
     1981 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     2041 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     2101 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     2161 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     2221 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     2281 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     2341 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagcattg agaaagcgcc
     2401 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     2461 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     2521 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     2581 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     2641 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     2701 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     2761 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     2821 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt
     2881 accgctagcc aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct
     2941 gcttagtttg atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt
     3001 gcttcacaac gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg
     3061 acaaacaaca gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat
     3121 gcctggcagt tccctactct cgcgttaacg ctagcatgga tgttttccca gtcacgacgt
     3181 tgtaaaacga cggccagtct taagctcggg ccc
//
