LOCUS       sequence-131837-        9469 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express FLPe and tdTomato under CB promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9469)
  TITLE     lenti vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCDH-CB-FLPe-P2A-tdTomato
FEATURES             Location/Qualifiers
     source          1..9469
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             234..414
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    458..583
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1076..1309
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1798..1915
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     intron          2567..2663
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             join(2741..2746,2747..2767,2768..4036)
                     /codon_start=1
                     /product="nuclear-targeted site-specific recombinase"
                     /label=FLPe
                     /note="FLPe is a fusion between the SV40 nuclear 
                     localization signal and a thermostable version of the 
                     Saccharomyces cerevisiae site-specific recombinase FLP. 
                     Recombination occurs at FRT sequences (Buchholz et al., 
                     1998)."
                     /translation="MAPKKKRKVMSQFDILCKTPPKVLVRQFVERFERPSGEKIASCAA
                     ELTYLCWMITHNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKK
                     LIPAWEFTIIPYNGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESI
                     WEITEKILNSFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGV
                     IIQCLVTETKTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEY
                     QLLKDNLVRSYNKALKKNAPYPIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWS
                     DKRASAVARTTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQL
                     KGSAEGSIRYPAWNGIISQEVLDYLSSYINRRI"
     CDS             4052..4108
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4118..5548
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     misc_feature    5564..6152
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5617..5637)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6035..6046)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6226..6459
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6531..6652
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6568..6587)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6622..6641
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6671..6806
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6844..6860)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6844..6860)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6857..6879)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6868..6884
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6892..6898,6899..6916,6917..6922))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6937..6958
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7075..7092)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7246..7834)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(8005..8796,8797..8865))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8628..8647
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8866..8970)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9038..9056
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9094..9116)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9216..9235
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9429..9451
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9443..9460
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9444..9460
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 ttaattaagg tacctctggt cgttacataa cttacggtaa atggcccgcc tggctgaccg
     1981 cccaacgacc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     2041 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     2101 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     2161 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctact
     2221 cgaggccacg ttctgcttca ctctccccat ctcccccccc tccccacccc caattttgta
     2281 tttatttatt ttttaattat tttgtgcagc gatgggggcg gggggggggg gggggggggc
     2341 gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg
     2401 cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc
     2461 ggcggcccta taaaaagcga agcgcgcggc gggcgggagc gggatcagcc accgcggtgg
     2521 cggccctaga gtcgatcgag gaactgaaaa accagaaagt taactggtaa gtttagtctt
     2581 tttgtctttt atttcaggtc cccgatccgg tggtggtgca aatcaaagaa ctgctcctca
     2641 gtggatgttg cctttacttc taggcctgta cggaagtgtt acttctgctc taaaagctgc
     2701 ggaattgtac ccgcggccga tccaccggtc gggatccacc atggctccca agaagaagag
     2761 gaaggtgatg agtcaatttg atatattatg taaaacacca cctaaggtcc tggttcgtca
     2821 gtttgtggaa aggtttgaaa gaccttcagg ggaaaaaata gcatcatgtg ctgctgaact
     2881 aacctattta tgttggatga ttactcataa cggaacagca atcaagagag ccacattcat
     2941 gagctataat actatcataa gcaattcgct gagtttcgat attgtcaaca aatcactcca
     3001 gtttaaatac aagacgcaaa aagcaacaat tctggaagcc tcattaaaga aattaattcc
     3061 tgcttgggaa tttacaatta ttccttacaa tggacaaaaa catcaatctg atatcactga
     3121 tattgtaagt agtttgcaat tacagttcga atcatcggaa gaagcagata agggaaatag
     3181 ccacagtaaa aaaatgctta aagcacttct aagtgagggt gaaagcatct gggagatcac
     3241 tgagaaaata ctaaattcgt ttgagtatac ctcgagattt acaaaaacaa aaactttata
     3301 ccaattcctc ttcctagcta ctttcatcaa ttgtggaaga ttcagcgata ttaagaacgt
     3361 tgatccgaaa tcatttaaat tagtccaaaa taagtatctg ggagtaataa tccagtgttt
     3421 agtgacagag acaaagacaa gcgttagtag gcacatatac ttctttagcg caaggggtag
     3481 gatcgatcca cttgtatatt tggatgaatt tttgaggaat tctgaaccag tcctaaaacg
     3541 agtaaatagg accggcaatt cttcaagcaa caaacaggaa taccaattat taaaagataa
     3601 cttagtcaga tcgtacaaca aggctttgaa gaaaaatgcg ccttatccaa tctttgctat
     3661 aaagaatggc ccaaaatctc acattggaag acatttgatg acctcatttc tgtcaatgaa
     3721 gggcctaacg gagttgacta atgttgtggg aaattggagc gataagcgtg cttctgccgt
     3781 ggccaggaca acgtatactc atcagataac agcaatacct gatcactact tcgcactagt
     3841 ttctcggtac tatgcatatg atccaatatc aaaggaaatg atagcattga aggatgagac
     3901 taatccaatt gaggagtggc agcatataga acagctaaag ggtagtgctg aaggaagcat
     3961 acgatacccc gcatggaatg ggataatatc acaggaggta ctagactacc tttcatccta
     4021 cataaataga cgcataaagc ttggaagcgg agctactaac ttcagcctgc tgaagcaggc
     4081 tggagacgtg gaggagaacc ctggacctcc cgggccaatg gtgagcaagg gcgaggaggt
     4141 catcaaagag ttcatgcgct tcaaggtgcg catggagggc tccatgaacg gccacgagtt
     4201 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
     4261 ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc agttcatgta
     4321 cggctccaag gcgtacgtga agcaccccgc cgacatcccc gattacaaga agctgtcctt
     4381 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggtc tggtgaccgt
     4441 gacccaggac tcctccctgc aggacggcac gctgatctac aaggtgaaga tgcgcggcac
     4501 caacttcccc cccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctccac
     4561 cgagcgcctg tacccccgcg acggcgtgct gaagggcgag atccaccagg ccctgaagct
     4621 gaaggacggc ggccactacc tggtggagtt caagaccatc tacatggcca agaagcccgt
     4681 gcaactgccc ggctactact acgtggacac caagctggac atcacctccc acaacgagga
     4741 ctacaccatc gtggaacagt acgagcgctc cgagggccgc caccacctgt tcctggggca
     4801 tggcaccggc agcaccggca gcggcagctc cggcaccgcc tcctccgagg acaacaacat
     4861 ggccgtcatc aaagagttca tgcgcttcaa ggtgcgcatg gagggctcca tgaacggcca
     4921 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     4981 gctgaaggtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt ccccccagtt
     5041 catgtacggc tccaaggcgt acgtgaagca ccccgccgac atccccgatt acaagaagct
     5101 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggtctggt
     5161 gaccgtgacc caggactcct ccctgcagga cggcacgctg atctacaagg tgaagatgcg
     5221 cggcaccaac ttcccccccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
     5281 ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc accaggccct
     5341 gaagctgaag gacggcggcc actacctggt ggagttcaag accatctaca tggccaagaa
     5401 gcccgtgcaa ctgcccggct actactacgt ggacaccaag ctggacatca cctcccacaa
     5461 cgaggactac accatcgtgg aacagtacga gcgctccgag ggccgccacc acctgttcct
     5521 gtacggcatg gacgagctgt acaagtaata agaattcgtc gacaatcaac ctctggatta
     5581 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     5641 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     5701 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     5761 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     5821 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     5881 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     5941 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
     6001 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     6061 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     6121 gagtcggatc tccctttggg ccgcctcccc gcctggtacc tttaagacca atgacttaca
     6181 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     6241 actcccaacg aaaataagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     6301 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     6361 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     6421 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
     6481 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     6541 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     6601 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     6661 ggctctagct atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     6721 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     6781 gtagtgagga ggcttttttg gaggcctaga cttttgcaga gacggcccaa attcgtaatc
     6841 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     6901 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     6961 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     7021 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     7081 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     7141 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     7201 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     7261 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     7321 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     7381 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     7441 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     7501 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     7561 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     7621 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     7681 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     7741 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     7801 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     7861 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     7921 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     7981 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     8041 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     8101 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     8161 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     8221 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     8281 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     8341 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     8401 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     8461 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     8521 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     8581 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     8641 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     8701 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     8761 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     8821 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     8881 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     8941 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     9001 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     9061 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     9121 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     9181 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
     9241 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     9301 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     9361 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     9421 agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctg
//
