LOCUS       sequence-131888-        6304 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  luciferase reporter with 3 copies of the stromelylisin gene AP-1 
            site in front of hGM-CSF -55 to +28 minimal promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6304)
  TITLE     Cockerill Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6304)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAP1-3
FEATURES             Location/Qualifiers
     source          1..6304
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      287..296
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             293..1945
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(359..377)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          2030..2095
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2225..2245
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2667..2801
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3572..3590)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3658..3762
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3763..3831,3832..4623)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3981..4000)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4794..5382
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5283..5302
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5536..5553
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5568..5708
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5634..5653)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5794..5816
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     polyA_signal    5947..6081
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    join(6175..6304,1..5)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 ggatccaagc tcagatctcg agatctgcaa ggatgagtca agctgcgggt gatccagatc
       61 tgcaaggatg agtcaagctg cgggtgatcc agatctgcaa ggatgagtca agctgcgggt
      121 gatccagatc tgggcccgga tcctgcaggg ggtcgacaag cttcgcgagg tcaccattaa
      181 tcatttcctc tgtgtattta agagctcttt tgccagtgag cccagtacac agagagaaag
      241 gctaaagttc tgtcgacaag cttggcattc cggtactgtt ggtaaagcca ccatggaaga
      301 cgccaaaaac ataaagaaag gcccggcgcc attctatccg ctggaagatg gaaccgctgg
      361 agagcaactg cataaggcta tgaagagata cgccctggtt cctggaacaa ttgcttttac
      421 agatgcacat atcgaggtgg acatcactta cgctgagtac ttcgaaatgt ccgttcggtt
      481 ggcagaagct atgaaacgat atgggctgaa tacaaatcac agaatcgtcg tatgcagtga
      541 aaactctctt caattcttta tgccggtgtt gggcgcgtta tttatcggag ttgcagttgc
      601 gcccgcgaac gacatttata atgaacgtga attgctcaac agtatgggca tttcgcagcc
      661 taccgtggtg ttcgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc aaaaaaagct
      721 cccaatcatc caaaaaatta ttatcatgga ttctaaaacg gattaccagg gatttcagtc
      781 gatgtacacg ttcgtcacat ctcatctacc tcccggtttt aatgaatacg attttgtgcc
      841 agagtccttc gatagggaca agacaattgc actgatcatg aactcctctg gatctactgg
      901 tctgcctaaa ggtgtcgctc tgcctcatag aactgcctgc gtgagattct cgcatgccag
      961 agatcctatt tttggcaatc aaatcattcc ggatactgcg attttaagtg ttgttccatt
     1021 ccatcacggt tttggaatgt ttactacact cggatatttg atatgtggat ttcgagtcgt
     1081 cttaatgtat agatttgaag aagagctgtt tctgaggagc cttcaggatt acaagattca
     1141 aagtgcgctg ctggtgccaa ccctattctc cttcttcgcc aaaagcactc tgattgacaa
     1201 atacgattta tctaatttac acgaaattgc ttctggtggc gctcccctct ctaaggaagt
     1261 cggggaagcg gttgccaaga ggttccatct gccaggtatc aggcaaggat atgggctcac
     1321 tgagactaca tcagctattc tgattacacc cgagggggat gataaaccgg gcgcggtcgg
     1381 taaagttgtt ccattttttg aagcgaaggt tgtggatctg gataccggga aaacgctggg
     1441 cgttaatcaa agaggcgaac tgtgtgtgag aggtcctatg attatgtccg gttatgtaaa
     1501 caatccggaa gcgaccaacg ccttgattga caaggatgga tggctacatt ctggagacat
     1561 agcttactgg gacgaagacg aacacttctt catcgttgac cgcctgaagt ctctgattaa
     1621 gtacaaaggc tatcaggtgg ctcccgctga attggaatcc atcttgctcc aacaccccaa
     1681 catcttcgac gcaggtgtcg caggtcttcc cgacgatgac gccggtgaac ttcccgccgc
     1741 cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg attacgtcgc
     1801 cagtcaagta acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg acgaagtacc
     1861 gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga gagatcctca taaaggccaa
     1921 gaagggcgga aagatcgccg tgtaattcta gaggatcttt gtgaaggaac cttacttctg
     1981 tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg taaatataaa
     2041 atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattcc
     2101 aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt
     2161 ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc aacattctac
     2221 tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag aattgctaag
     2281 ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta tttacaccac
     2341 aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatattctg taacctttat
     2401 aagtaggcat aacagttata atcataacat actgtttttt cttactccac acaggcatag
     2461 agtgtctgct attaataact atgctcaaaa attgtgtacc tttagctttt taatttgtaa
     2521 aggggttaat aaggaatatt tgatgtatag tgccttgact agagatcata atcagccata
     2581 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     2641 aacataaaat gaatgcaatt gttgttaact tgtttattgc agcttataat ggttacaaat
     2701 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     2761 gtttgtccaa actcatcaat gtatcttatc atgtctggat cgatccccag gaagctcctc
     2821 tgtgtcctca taaaccctaa cctcctctac ttgagaggac attccaatca taggctgccc
     2881 atccaccctc tgtgtcctcc tgttaattag gtcacttaac aaaaaggaaa ttgggtaggg
     2941 gtttttcaca gaccgctttc taagggtaat tttaaaatat ctgggaagtc ccttccactg
     3001 ctgtgttcca gaagtgttgg taaacagccc acaaatgtca acagcagaaa catacaagct
     3061 gtcagctttg cacaagggcc caacaccctg ctcatcaaga agcactgtgg ttgctgtgtt
     3121 agtaatgtgc aaaacaggag gcacattttc cccacctgtg taggttccaa aatatctagt
     3181 gttttcattt ttacttggat caggaaccca gcactccact ggataagcat tatccttatc
     3241 caaaacagcc ttgtggtcag tgttcatctg ctgactgtca actgtagcat tttttggggt
     3301 tacagtttga gcaggatatt tggtcctgta gtttgctaac acaccctgca gctccaaagg
     3361 ttccccacca acagcaaaaa aatgaaaatt tgacccttga atgggttttc cagcaccatt
     3421 ttcatgagtt ttttgtgtcc ctgaatgcaa gtttaacata gcagttaccc caataacctc
     3481 agttttaaca gtaacagctt cccacatcaa aatatttcca caggttaagt cctcatttaa
     3541 attaggcaaa ggaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt
     3601 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     3661 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     3721 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     3781 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     3841 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     3901 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     3961 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa
     4021 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     4081 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     4141 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     4201 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     4261 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca
     4321 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     4381 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     4441 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     4501 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     4561 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     4621 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     4681 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     4741 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     4801 cctttttttc tgcgcgtaat atgctgcttg caaacaaaaa aaccaccgct accagcggtg
     4861 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     4921 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     4981 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     5041 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     5101 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     5161 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     5221 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     5281 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     5341 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     5401 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     5461 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     5521 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat
     5581 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
     5641 tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca
     5701 tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     5761 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     5821 caccgtcatc accgaaacgc gcgaggcagg atcagccata tcacatttgt agaggtttta
     5881 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     5941 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     6001 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     6061 gtatcttatc atgtctggat cagccatatc acatttgtag aggttttact tgctttaaaa
     6121 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttaacttg
     6181 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     6241 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     6301 gtct
//
