LOCUS       sequence-131908-         759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression plasmid for SaCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Kleinstiver BP, Prew MS, Tsai SQ, Nguyen NT, Topkar VV, Zheng Z, 
            Joung JK
  TITLE     Broadening the targeting range of Staphylococcus aureus CRISPR-Cas9 
            by modifying PAM recognition.
  JOURNAL   Nat Biotechnol. 2015 Nov 2. doi: 10.1038/nbt.3404.
  PUBMED    26524662
REFERENCE   2  (bases 1 to 759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Nov 2. doi: 10.1038/nbt.3404."
COMMENT     Sequence Label: MSP2266
FEATURES             Location/Qualifiers
     source          1..759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     106..125
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        106..124
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    125..149
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 actctcttcc gggcgctatc atgccatacc gcgaaaggtt ttgcgccatt cgatggtgtc
       61 cgggatctcg acgctctccc ttatgcgact cctgcattag gaaattaata cgactcacta
      121 taggggaatt gtgagcggat aacaattccc ctgtagaaat aattttgttt aactttaata
      181 aggagatata ccatgggcaa acggaactac atcctggggc ttgacattgg gataaccagc
      241 gttggctacg gaattattga ttatgagaca cgcgatgtga ttgacgccgg ggttaggctg
      301 ttcaaagagg ccaacgttga aaacaacgag ggaagacgga gtaagcgcgg agcaagaaga
      361 ctcaagcgca gacggagaca tcggattcag agggtgaaaa agctgctctt cgattacaat
      421 ctcctgaccg atcatagtga gctgagcgga atcaacccct acgaggcgcg agtgaaaggg
      481 ctttcccaga agctgtccga agaggagttc tccgccgcgt tgctgcacct ggccaaacgg
      541 aggggggttc acaatgtaaa cgaagtggag gaggacacgg gcaatgaact tagtacgaaa
      601 gaacagatca gtaggaactc taaggctctc gaagagaaat acgtcgctga gttgcagctt
      661 gagagactga aaaaagacgg cgaagtacgc ggatctatta ataggttcaa gacttcagat
      721 tacgtaaagg aagccaagca gctcctgaaa gtacagaaa
//
