LOCUS       sequence-131998-         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Homologues recombination donor plasmid for inserting a FLAG-SNAP-tag
            at the N-terminus of the TERT locus in human cells. Includes PURO 
            resistance flanked by LoxP sites for selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Xi L, Schmidt JC, Zaug AJ, Ascarrunz DR, Cech TR
  TITLE     A novel two-step genome editing strategy with CRISPR-Cas9 provides 
            new insights into telomerase action and TERT gene expression.
  JOURNAL   Genome Biol. 2015 Nov 10;16(1):231. doi: 10.1186/s13059-015-0791-1.
  PUBMED    26553065
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2015-11-10"; volume: "16(1):231. doi"; pages: " 
            10.1186/s13059-015-0791-1"
COMMENT     Sequence Label: FLAG-LoxP-PURO-LoxP-SNAP-TERT
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    294..327
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             336..881
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="optimized for bacterial expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHEIKLLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYQQLAALAGNPAATAAVKTALSGNPVPILIPCHRVVSSSGAVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
ORIGIN
        1 tcctcactac ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa
       61 aaaaattagt cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat
      121 gggcggagtt aggggcggga ctatggttgc tgactaattg agatgcatgc tttgcatact
      181 tctgcctgct ggggagcctg gggactttcc acacctggtt gctgactaat tgagatgcat
      241 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacaccgga tccataactt
      301 cgtataatgt atgctatacg aagttatccg gtaccatgga caaagattgc gaaatgaaac
      361 gtaccaccct ggatagcccg ctgggcaaac tggaactgag cggctgcgaa cagggcctgc
      421 atgaaattaa actgctgggt aaaggcacca gcgcggccga tgcggttgaa gttccggccc
      481 cggccgccgt gctgggtggt ccggaaccgc tgatgcaggc gaccgcgtgg ctgaacgcgt
      541 attttcatca gccggaagcg attgaagaat ttccggttcc ggcgctgcat catccggtgt
      601 ttcagcagga gagctttacc cgtcaggtgc tgtggaaact gctgaaagtg gttaaatttg
      661 gcgaagtgat tagctatcag cagctggcgg ccctggcggg taatccggcg gccaccgccg
      721 ccgttaaaac cgcgctgagc ggtaacccgg tgccgattct gattccgtgc catcgtgtgg
      781 ttagctctag cggtgcggtt ggcggttatg aaggtggtct ggcggtgaaa gagtggctgc
      841 tggcccatga aggtcatcgt ctgggtaaac cgggtctggg acctgcaggt ataggcgcgc
      901 caggatccgg taccatgccg cgcgctcc
//
