LOCUS       sequence-132000-         572 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Homologues recombination donor plasmid for inserting a FLAG-SNAP-tag
            at the N-terminus of the TERT locus in human cells. Includes PURO 
            resistance flanked by LoxP sites for selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Xi L, Schmidt JC, Zaug AJ, Ascarrunz DR, Cech TR
  TITLE     A novel two-step genome editing strategy with CRISPR-Cas9 provides 
            new insights into telomerase action and TERT gene expression.
  JOURNAL   Genome Biol. 2015 Nov 10;16(1):231. doi: 10.1186/s13059-015-0791-1.
  PUBMED    26553065
REFERENCE   2  (bases 1 to 572)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2015-11-10"; volume: "16(1):231. doi"; pages: " 
            10.1186/s13059-015-0791-1"
COMMENT     Sequence Label: FLAG-LoxP-PURO-LoxP-SNAP-TERT
FEATURES             Location/Qualifiers
     source          1..572
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ggatgggtgg gagtggcgcg tgccagagag cgcaccctcc aaagaggtgg cttcttcggc
       61 gggtctggca ggtgacacca cacagaaacc acggtcactc ggtccacgcg tcctgcccgg
      121 gtgggcccag gacccctgcc caacgggcgt ccgctccggc tcaggggcag cgccacgcct
      181 gggcctcttg ggcaacggca gacttcggct ggcactgccc ccgcgcctcc tcgcacccgg
      241 ggctggcagg cccaggggga ccccggcctc cctgacgcta tggttccagg cccgttcgca
      301 tcccagacgc cttcggggtc cactagcgtg tggcgggggc cgggcctgag tggcagcgcc
      361 gagctggtac agcggcggcc cgcacacctg gtaggcgcag ctgggagcca ccagcacaaa
      421 gagcgcgcag cgtgccagca ggtgaaccag cacgtcgtcg cccacgcggc gcagcagcag
      481 cccccacgcc ccgctccccc gcagtgcgtc ggtcaccgtg ttgggcaggt agctgcgcac
      541 gctggtggtg aaggcctcgg gggggccccc gc
//
