LOCUS       sequence-132004-         748 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Homologues recombination donor plasmid for inserting a FLAG-SNAP-tag
            at the N-terminus of the TERT locus in human cells. Includes PURO 
            resistance flanked by LoxP sites for selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Xi L, Schmidt JC, Zaug AJ, Ascarrunz DR, Cech TR
  TITLE     A novel two-step genome editing strategy with CRISPR-Cas9 provides 
            new insights into telomerase action and TERT gene expression.
  JOURNAL   Genome Biol. 2015 Nov 10;16(1):231. doi: 10.1186/s13059-015-0791-1.
  PUBMED    26553065
REFERENCE   2  (bases 1 to 748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2015-11-10"; volume: "16(1):231. doi"; pages: " 
            10.1186/s13059-015-0791-1"
COMMENT     Sequence Label: FLAG-LoxP-PURO-LoxP-SNAP-TERT
FEATURES             Location/Qualifiers
     source          1..748
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    31..79
                     /note="synthetic polyadenylation signal"
     protein_bind    complement(86..119)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(120..185)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 tggtgcatga cccgcaagcc cggtgcctga aataaaatat ctttattttc attacatctg
       61 tgtgttggtt ttttgtgtga agcttataac ttcgtatagc atacattata cgaagttatc
      121 ttgtcatcgt catccttgta atcgatatca tgatctttat aatcaccgtc atggtctttg
      181 tagtccatcg cgggggtggc cggggccagg gcttcccacg tgcgcagcag gacgcagcgc
      241 tgcctgaaac tcgcgccgcg aggagagggc ggggccgcgg aaaggaaggg gaggggctgg
      301 gagggcccgg agggggctgg gccggggacc cgggaggggt cgggacgggg cggggtccgc
      361 gcggaggagg cggagctgga aggtgaaggg gcaggacggg tgcccgggtc cccagtccct
      421 ccgccacgtg ggaagcgcgg tcctgggcgt ctgtgcccgc gaatccactg ggagcccggc
      481 ctggccccga cagcgcagct gctccgggcg gacccggggg tctgggccgc gcttccccgc
      541 ccgcgcgccg ctcgcgctcc cagggtgcag ggacgccagc gagggcccca gcggagagag
      601 gtcgaatcgg cctaggctgt ggggtaaccc gagggagggg ccatgatgtg gaggccctgg
      661 gaacaggtgc gtgcggcgac cctttggccg ctggcctgat ccggagaccc agggctgcct
      721 ccaggtccgg acgcggggcg tcgggctc
//
