LOCUS       sequence-132332-         711 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytosolic FRET, Excitation: 504 / 554, Emission: 517 /
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Davidson Lab
  TITLE     FRET Indicators - Live and Fixed Cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: mPA-GFP-tdTomato-FRET-10
FEATURES             Location/Qualifiers
     source          1..711
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(168..186)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     436..455
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     630..653
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 accgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggaggtcat
       61 caaagagttc atgcgcttca aggtgcgcat ggagggctcc atgaacggcc acgagttcga
      121 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
      181 gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tccccccagt tcatgtacgg
      241 ctccaaggcg tacgtgaagc accccgccga catccccgat tacaagaagc tgtccttccc
      301 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac
      361 ccaggactcc tccctgcagg acggcacgct gatctacaag gtgaagatgc gcggcaccaa
      421 cttccccccc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga
      481 gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa
      541 ggacggcggc cactacctgg tggagttcaa gaccatctac atggccaaga agcccgtgca
      601 actgcccggc tactactacg tggacaccaa gctggacatc acctcccaca acgaggacta
      661 caccatcgtg gaacagtacg agcgctccga gggccgccac cacctgttcc t
//
