LOCUS       sequence-132438-         672 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to make mitf knockout porcine cell line.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Wang X, Zhou J, Cao C, Huang J, Hai T, Wang Y, Zheng Q, Zhang H, Qin
            G, Miao X, Wang H, Cao S, Zhou Q, Zhao J
  TITLE     Efficient CRISPR/Cas9-mediated biallelic gene disruption and 
            site-specific knockin after rapid selection of highly active sgRNAs 
            in pigs.
  JOURNAL   Sci Rep. 2015 Aug 21;5:13348. doi: 10.1038/srep13348.
  PUBMED    26293209
REFERENCE   2  (bases 1 to 672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-08-21"; volume: "5:13348. doi"; pages: " 
            10.1038/srep13348"
COMMENT     Sequence Label: pX330-mitf
FEATURES             Location/Qualifiers
     source          1..672
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             14..37
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             44..64
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggatcatgat attgattaca aagacgatga cgataagatg gccccaaaga agaagcggaa
       61 ggtcggtatc cacggagtcc cagcagccga caagaagtac agcatcggcc tggacatcgg
      121 caccaactct gtgggctggg ccgtgatcac cgacgagtac aaggtgccca gcaagaaatt
      181 caaggtgctg ggcaacaccg accggcacag catcaagaag aacctgatcg gagccctgct
      241 gttcgacagc ggcgaaacag ccgaggccac ccggctgaag agaaccgcca gaagaagata
      301 caccagacgg aagaaccgga tctgctatct gcaagagatc ttcagcaacg agatggccaa
      361 ggtggacgac agcttcttcc acagactgga agagtccttc ctggtggaag aggataagaa
      421 gcacgagcgg caccccatct tcggcaacat cgtggacgag gtggcctacc acgagaagta
      481 ccccaccatc taccacctga gaaagaaact ggtggacagc accgacaagg ccgacctgcg
      541 gctgatctat ctggccctgg cccacatgat caagttccgg ggccacttcc tgatcgaggg
      601 cgacctgaac cccgacaaca gcgacgtgga caagctgttc atccagctgg tgcagaccta
      661 caaccagctg tt
//
