LOCUS       sequence-132446-         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression; in vitro transcription/translation (T7).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Grou CP, Pinto MP, Mendes AV, Domingues P, Azevedo JE
  TITLE     The de novo synthesis of ubiquitin: identification of 
            deubiquitinases acting on ubiquitin precursors.
  JOURNAL   Sci Rep. 2015 Aug 3;5:12836. doi: 10.1038/srep12836.
  PUBMED    26235645
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-08-3"; volume: "5:12836. doi"; pages: " 
            10.1038/srep12836"
COMMENT     Sequence Label: pET23a-Mm/HsUBA52
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..46)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(252..479)
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     RBS             487..509
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(540..559)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(541..559)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ttcgggcttt gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg agtgcggccg
       61 caagcttgtc gacggagctc gaattcggat ccttatttga ccttcttctt gggacgcagg
      121 ttgttggtgt gaccacactt cttcttgcgg cagttgacag cacgagggtg aaggcgagca
      181 tagcacttgc ggcagatcat cttgtcgcag ttgtatttct gggcaagctg gcggagagaa
      241 ggctcaataa tgccacctcg caggcgcaac accaggtgca gggtggactc tttctggatg
      301 ttgtagtctg agagagtgcg gccatcctcc agctgtttgc cggcaaatat cagacgctgc
      361 tggtcaggtg ggataccctc cttgtcttga attttggctt tgacattctc aatggtgtca
      421 ctgggctcga cctcaagggt gatggttttg ccagtgaggg tcttcacaaa gatctgcata
      481 tgtatatctc cttcttaaag ttaaacaaaa ttatttctag agggaaaccg ttgtggtctc
      541 cctatagtga gtcgtattaa tttcgcggga tcgagatctc gggcagcgtt gggtcctggc
      601 cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc
      661 ttactggtta gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca
      721 aaacgtctgc gacctgagca acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc
      781 tggaaacgcg gaagtcagcg ccctgcacca ttatgttccg gatctgcatc gc
//
