LOCUS       sequence-132515-         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes human full length SENP6 optimised for E. coli expression. 
            Suitable for in vitro transcription/translation (T7)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Mendes AV, Grou CP, Azevedo JE, Pinto MP
  TITLE     Evaluation of the activity and substrate specificity of the human 
            SENP family of SUMO proteases.
  JOURNAL   Biochim Biophys Acta. 2015 Oct 29. pii: S0167-4889(15)00373-0. doi: 
            10.1016/j.bbamcr.2015.10.020.
  PUBMED    26522917
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochim 
            Biophys Acta. 2015 Oct 29. pii: S0167-4889(15)00373-0. doi: 
            10.1016/j.bbamcr.2015.10.020."
COMMENT     Sequence Label: pET28a-HsSENP6
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             44..61
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             71..88
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 attttgttta actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca
       61 cagcagcggc ctggtgccgc gcggcagcca tatggcggcg ggcaaatcgg gcggctcggc
      121 gggcgaaatc acctttctgg aagcactggc acgcagcgaa tcaaaacgcg acggcggctt
      181 caaaaacaac tggagtttcg atcatgaaga agaatccgaa ggcgacaccg ataaagacgg
      241 tacgaacctg ctgtccgtgg atgaagacga agattccgaa acctcaaaag gcaaaaaact
      301 gaaccgtcgc tcagaaattg tcgcgaatag ctctggtgaa tttatcctga aaacctatgt
      361 gcgtcgcaat aaatcggaaa gcttcaaaac gctgaaaggc aacccgattg gtctgaatat
      421 gctgagcaac aataaaaaac tgtctgaaaa cacccagaat acgtcgctgt gcagcggcac
      481 cgtggttcat ggtcgtcgct ttcatcacgc acacgctcag atcccggtcg tgaaaacggc
      541 ggcccaaagt tccctggatc gtaaagaacg caaagaatat ccgccgcacg tgcagaaagt
      601 tgaaattaac ccggttcgtc tgagtcgcct gcaaggcgtc gaacgcatca tgaagaaaac
      661 cgaagaatct gaaagtcagg tggaaccgga aattaaacgc aaagttcagc aaaaacgtca
      721 ttgtagcacc tatcagccga cgccgccgct gtctccggca agtaaaaaat gcctgaccca
      781 cctggaagat ctgcagcgta actgtcgcca agctattacc ctgaatgaaa gcacgggtcc
      841 gctgctgcgt acctcgatc
//
