LOCUS       sequence-132518-        1072 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ampicillin resistant derivative of pDONR221 P1-P4 for multisite 
            GATEWAY cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Gu Y, Innes RW
  TITLE     The KEEP ON GOING protein of Arabidopsis recruits the ENHANCED 
            DISEASE RESISTANCE1 protein to trans-Golgi network/early endosome 
            vesicles.
  JOURNAL   Plant Physiol. 2011 Apr;155(4):1827-38. doi: 10.1104/pp.110.171785. 
            Epub 2011 Feb 22.
  PUBMED    21343429
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.110.171785"; journalName: "Plant Physiol"; date: 
            "2011-04"; volume: "155"; issue: "4"; pages: "1827-38"
COMMENT     Sequence Label: pBSDONR P1-P4
FEATURES             Location/Qualifiers
     source          1..1072
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(49..151)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    complement(296..527)
                     /gene="mutant version of attP"
                     /label=attP4
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(577..593)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(577..593)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(630..650)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(630..648)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(669..685)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(669..685)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(682..704)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    693..709
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(717..723,724..741,742..747))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    762..783
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(900..917)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt
       61 agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg
      121 gtgaaagttg gaacctctta cgtgccgatc aacgtctcat tttcgccaaa agttggccca
      181 gggcttcccg gtatcaacag ggacaccagg atttatttat tctgcgaagt gatcttccgt
      241 cacaggtatt tattcggcgc aaagtgcgtc gggtgatgct gccaacttag tcgactacag
      301 gtcactaata ccatctaagt agttgattca tagtgactgg atatgttgtg ttttacagta
      361 ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta
      421 cgtttctcgt tcaacttttc tatacaaagt tggcattata agaaagcatt gcttatcaat
      481 ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctgc
      541 agggcggccg cgatatcgaa ttcctgcagc ccgggggatc cactagttct agagcggccg
      601 ccaccgcggt ggagctccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg
      661 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
      721 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
      781 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
      841 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
      901 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
      961 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     1021 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tt
//
