LOCUS       sequence-13262-e        6275 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6275)
  AUTHORS   Gumbiner-Russo LM, Lombardo MJ, Ponder RG, Rosenberg SM
  TITLE     The TGV transgenic vectors for single-copy gene expression from the 
            Escherichia coli chromosome.
  JOURNAL   Gene. 2001 Jul 25. 273(1):97-104.
  PUBMED    11483365
REFERENCE   2  (bases 1 to 6275)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene. 2001 
            Jul 25. 273(1):97-104."
COMMENT     Sequence Label: pTGV-Light SMR5452
FEATURES             Location/Qualifiers
     source          1..6275
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(4296..4313)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4467..5055)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4547..4566)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5226..6017,6018..6086))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5849..5868
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6087..6191)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     join(6259..6275,1..2)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gccctggcgc gcctaggcca tatgggcccg ggcgagcata ttgatccgct gcaaactgaa
       61 attggcagca tggtctatgc ggtgaaacca ggcgatggtt ctgcgcgtga acaggcggcg
      121 agctgccagc gtgtgattgg cggtctggcg aatattgccg aggagtacgc gactaaacgc
      181 tatcgttcta acgtcatcaa ctgggggatg ttaccgctgc aaatggcgga agtaccaacc
      241 tttgaagtgg gggattacat ttacatccct ggcattaaag cggcgctgga taatccgggt
      301 acgacgttta aaggttatgt gatccatgaa gatgcgccgg taacggaaat tacgctctat
      361 atggaaagtc tgactgctga agagcgcgag attatcaagg cgggtagttt gattaacttc
      421 aataaaaacc gtcagatgta aaaagcgcca tgtgaatgta ggtcgcattc ggcacttatt
      481 gtcggatgcg atgcttgcgc atcttatccg acctacgaat cgcatcgaat ctgtaggcca
      541 gataaggcat tttcgcagca tccggcactt attgtcggat gcgatgcttg cgcatcttat
      601 ccgacctaca aatcgcatcg aaccgtaggc cggataaggc gtttacgccg catccggcaa
      661 atagttaatt gctcttactt cttcgcctct gcaaccactt tactacccac gccgcggtta
      721 ttgtattccc acatgcggtt gtagttagtg tcattcagat tgcgctgtat ttcgtcgtta
      781 tcatctacgc tgccggtatt acccgcaaac ggacgattag agatcaccgc atcggcccac
      841 ggtttagccg tgttaaaacc ttcgttgatg gcgctatcac ggatcaccac ctgaccgttg
      901 gtattggcat caacatccag cgagcggccc agttgcgcca caccatcacc gaaagcattg
      961 aaacggctgt ttacggcgag gaaaccgtag taaatgttgg acagcgtagc cggtgcaaac
     1021 acatacgctt cttgctgagt acgtgagttc accacgcgga attcggtgtt atcgaacacc
     1081 actgcgccgc gaccagaaac gatatccaca tccccttcaa tgtagctgtt ggtcaccagc
     1141 gtacgcggct gacgattcgt ttccagacgg ttctgcacac cgctgttggt gacaaagaag
     1201 gtgttctgac gaccgagaat gttaacgttg ttaatctgta cctggtcacc atcagtacgc
     1261 agtgccaccg ccggatggtt acctgcatct acgctatcgc ccagcgtgtt ttcgatggtc
     1321 agattttgca gttgcaggcc attgttttgt gaccagaaga ccgcagagca gagaacaccg
     1381 atactgtcgc tgcgtttgct ctggcagcta tcgtacatat accacgctgg tttacctggc
     1441 atatatttgc cgcgcgggtt gacgtcgtga cgccagtcgg cagggctcat gccaccatca
     1501 agggaaagcc caatcttcac atcaatcggt ttttcacctg taccgtacag agtaattcca
     1561 cccggagcgg cagggacata taccgttccc tgatactcac caggcatcac ggcaatatac
     1621 tggcgcttgt tggtacgctt gataattgcc gcatctaccg ccgcctgaat cgtggtatgc
     1681 gttacacctt gagtgcccgc cgggccgaca acaaagtcag gttgcgcagg cagggtaatc
     1741 ggggaaggat tccacgctgc agcacctggt gtcagggatg caaaatagtg ttgagcatcg
     1801 aaattctgcg cttcttttgc cgacagaatc gggcgagaag aggtaccagg cgcggtttga
     1861 tcagaaggac gttgatcggg cggggttgag ctacaggcgg tcagcgtcac gccaaaagcc
     1921 aatgccagcg ccagacggga aactgaaaat gtgttcacag gttgctccgg gctatgaaat
     1981 agaaaaatga atccgttgaa ggcatgcgtc tagagctcga ggtagatctg aaagcttgaa
     2041 tcgatgtacg tactataccg gtgcgaaacg ggaaggtaaa aagacaaaaa gttgttttta
     2101 atacctttaa gtgataccag atggcattgc gccatctggc agagtgatta actaaacatc
     2161 gcagtaatcg aggcgcttgc cagagagtgg aaatgaacgt taaacccgac catcgcgccg
     2221 ctggcacctt catcgacatc aatacgttct atatccagcg cgtgaacggt aaaaatgtag
     2281 cgatgagttt cgcctttcgg cggtgctgcg ccatcgtacc cggttttacc aaagtcggta
     2341 cgcgtctgca aaacgccgtc tggcattgct accagaccag agccaaaccc ttgcggtaat
     2401 acgcgggtat cagcgggtaa gttaacaact acccagtgcc accagccgga gccggttggc
     2461 gcatccgggt cgtagcaggt gacaacaaaa cttttcgttc ccgcaggaac atcatcccac
     2521 gccagatgcg gtgaaatatt atcgccatcg taacccatgc cgttaaagac atgacgatgc
     2581 ggcaatttat cgccatcgcg cagatcgtta ctgatgagtt tcatgaaccc tcctttcttg
     2641 tttgcagaaa gtgtagccag aaaccctcac gcggacttct cgttattggc aaaaaaatgt
     2701 ttcatcctgt accgcgcggt taaccgctgc ggtcagacgc tgcaactgtt gcgggagaat
     2761 aatatagggc ggcatcaggt aaatcagttt gccaaaaggc cggatccaga caccctgttc
     2821 gacaaagaat ttttgcagcg ccgccatatt caccggatga gtggtttcga ccacgccaat
     2881 ggcccccagt acgcgcacat cggcaaccat ttcggcatca cgggcggggg caagttgctc
     2941 gcgcagctgt acttcaatat ccgccacctg ttgctgccag tcgccagatt cgagaatcgc
     3001 caggctggcg tttgctgccg cgcaggccag cggattgccc ataaaagttg gcccatgcat
     3061 aaagcaaccg gcttcaccgt tactgatggt ttctgcaacc tcgcgcgtgg tgagtgtggc
     3121 ggaaagggtc attgtgccgc cggttaaggc tttaccgagg cacaaaatgt ccggcgcgat
     3181 ttctgcatgt tcacaggcaa acagtttccc ggtacgacca aatccagtgg cgatctcgtc
     3241 ggcaatcagc aagatacctt cgcgatcgca tattttgcgg attcgtttta accattccgg
     3301 atggtacatg cgcatcccgc ctgcgccctg gacaatcggc tcaatgatca ccgccgcgat
     3361 ttcatgacga tgcgccgcca tcaggcgggc aaagcccacc atatcgcgct catcccattc
     3421 gccatccatg cggctttgcg gggcgggagc aaacaggttt tctggcaggt agcctttcca
     3481 cagactgtgc attgagttat ccggatcgca caccgacatc gcgccaaagg tatcgccatg
     3541 ataaccattg cggaaggtca gaaaacgctg gcgcgcttcg cctttggctt gccagtactg
     3601 caacgccatt ttcatcgcca cttccaccgc tacggaaccg gagtccgcga gaaaaacgca
     3661 ctccagcggt tgcggcgtca tcgccaccag tttgcggcac agctcaatgg ctggcgcatg
     3721 ggtgataccg ccaaacatca catgcgacat ggcatcaatt tgcgacttca tcgccgcatt
     3781 aagctgcggg tgattgtagc cgtggatcgc cgcccaccag gacgacatac cgtcaaccag
     3841 gcgtctgccg tcagacaaaa tcagctcgca accttcggcg ctcaccaccg gataaaccgg
     3901 cagaggggag gtcatggatg tgtatgggtg ccagatatgg cgttggtcaa aggcaagatc
     3961 gtccgttgtc ataatcgact tgtaaaccaa attgaaaaga tttaggttta caagtctaca
     4021 ccgaattaac aacaaaaaac acgttttgga gaagccccat ggctcaccgc ccacgctgga
     4081 cattgtcgca agtcacagaa ttatttgaaa aaccgttgct ggatctgctg tttgaagcgc
     4141 agcaggtgca tcgccagcat ttcgatcctc gtcaggtgca ggtcagcacg ttgctgcata
     4201 tggcgcgcct aggccaattg ggcccgggca tatgggtgtg aaataccgca cagatgcgta
     4261 aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     4321 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     4381 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     4441 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     4501 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     4561 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     4621 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     4681 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     4741 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     4801 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     4861 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     4921 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     4981 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     5041 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     5101 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     5161 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     5221 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     5281 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     5341 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     5401 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     5461 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     5521 cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     5581 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     5641 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     5701 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     5761 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     5821 agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa
     5881 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     5941 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     6001 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     6061 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     6121 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     6181 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     6241 atgacattaa cctataaaaa taggcgtatc acgag
//
