LOCUS       sequence-132638-        1507 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  overexpression, codon optimized for mammalian expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Chantranupong L, Wolfson RL, Orozco JM, Saxton RA, Scaria SM, 
            Bar-Peled L, Spooner E, Isasa M, Gygi SP, Sabatini DM
  TITLE     The Sestrins interact with GATOR2 to negatively regulate the 
            amino-acid-sensing pathway upstream of mTORC1.
  JOURNAL   Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1016/j.celrep.2014.09.014. 
            Epub 2014 Sep 25.
  PUBMED    25263562
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2014.09.014"; journalName: "Cell Rep"; date: 
            "2014-10-9- 9"; volume: "9"; issue: "1"; pages: "1-8"
COMMENT     Sequence Label: pRK5-FLAG-Sestrin3
FEATURES             Location/Qualifiers
     source          1..1507
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gcgtcgacga tgaatcgggg gggggggtca ccatccgccg ccgccaacta tctgctgtgc
       61 actaactgta gaaaagtcct gagaaaagac aaacggatcc gcgtgagtca gccactgaca
      121 cgaggcccca gcgccttcat tcctgagaag gaagtggtcc aggctaacac cgtggatgag
      181 agaacaaatt ttctggtcga ggaatacagc acttccggga ggctggacaa catcactcag
      241 gtcatgagcc tgcataccca gtacctggag tcattcctgc ggagccagtt ttatatgctg
      301 agaatggatg gacccctgcc tctgccatac cgacactata tcgccattat ggccgctgca
      361 cggcaccagt gcagctacct gatcaatatg catgtggacg agttcctgaa gacaggcggg
      421 attgccgagt ggctgaacgg cctggaatat gtgcctcagc ggctgaagaa cctgaatgag
      481 atcaacaagc tgctggccca cagaccatgg ctgatcacca aagaacatat tcagaagctg
      541 gtgaaaacag gggagaacaa ttggtccctg cccgaactgg tccacgcagt ggtcctgctg
      601 gcccactacc atgccctggc tagtttcgtg tttggatcag gcatcaaccc cgagagggat
      661 cctgaaatct caaatggctt ccgcctgatt agcgtgaaca atttttgcgt ctgtgacctg
      721 gctaacgata acaatatcga gaatgcatct ctgagtggct caaacttcgg gattgtggat
      781 agcctgtccg agctggaagc cctgatggag cgaatgaagc ggctgcagga ggaacgcgaa
      841 gacgaggaag ctagccagga ggaaatgtcc acccgattcg agaaagaaaa gaaagagtct
      901 ctgtttgtgg tcagtggcga tactttccac tcctttcctc attctgactt tgaagacgat
      961 atgatcatta cctctgacgt gagtaggtac atcgaggatc cagggttcgg atatgaagac
     1021 tttgcacgga gaggcgagga acacctgccc acattccgcg cccaggacta cacttgggag
     1081 aaccatgggt tttccctggt gaataggctg tattctgaca ttggacacct gctggatgag
     1141 aagttccgca tggtctacaa cctgacctat aatacaatgg ctactcatga agacgtggat
     1201 accacaatgc tgaggcgcgc actgttcaac tacgtccact gcatgtttgg aatccggtat
     1261 gacgattacg attatggcga agtgaatcag ctgctggaaa gatctctgaa ggtgtacatc
     1321 aagacagtca cttgttatcc cgagagaact accaaaagga tgtacgactc ctattggcgg
     1381 cagtttaagc actctgagaa agtgcatgtc aacctgctgc tgatggaagc aagaatgcag
     1441 gctgaactgc tgtatgctct gagggctatt actcgccatc tgacctaacg cggccgcttt
     1501 tttcctt
//
