LOCUS       sequence-132845-        1046 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of tagRFP targeted to the mitochondrial matrix.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Mishra P, Carelli V, Manfredi G, Chan DC
  TITLE     Proteolytic cleavage of Opa1 stimulates mitochondrial inner membrane
            fusion and couples fusion to oxidative phosphorylation.
  JOURNAL   Cell Metab. 2014 Apr 1;19(4):630-41. doi: 
            10.1016/j.cmet.2014.03.011.
  PUBMED    24703695
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2014.03"; journalName: "Cell Metab"; date: 
            "2014-04-1- 1"; volume: "19"; issue: "4"; pages: "630-41"
COMMENT     Sequence Label: pclbw-mitoTagRFP
FEATURES             Location/Qualifiers
     source          1..1046
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     19..38
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    99..123
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             130..204
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             226..954
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="VSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMR
                     IKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGV
                     LTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTDM
                     ALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVA
                     RYCDLPSKLGHKLNGMDELYK"
     protein_bind    complement(958..982)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag
       61 aattgatttg ataccgcggg cccgggatcc ctcgaatcac aagtttgtac aaaaaagcag
      121 gctgacacca tgtccgtcct gacgccgctg ctgctgcggg gcttgacagg ctcggcccgg
      181 cggctcccag tgccgcgcgc caagatccat tcgttggggg atccggtgtc taagggcgaa
      241 gagctgatta aggagaacat gcacatgaag ctgtacatgg agggcaccgt gaacaaccac
      301 cacttcaagt gcacatccga gggcgaaggc aagccctacg agggcaccca gaccatgaga
      361 atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg acatcctggc taccagcttc
      421 atgtacggca gcagaacctt catcaaccac acccagggca tccccgattt ctttaagcag
      481 tccttccctg agggcttcac atgggagaga gtcaccacat acgaagacgg gggcgtgctg
      541 accgctaccc aggacaccag cctccaggac ggctgcctca tctacaacgt caagatcaga
      601 ggggtgaact tcccatccaa cggccctgtg atgcagaaga aaacactcgg ctgggaggcc
      661 aacaccgaga tgctgtaccc cgctgacggc ggcctggaag gcagaaccga catggccctg
      721 aagctcgtgg gcgggggcca cctgatctgc aacttcaaga ccacatacag atccaagaaa
      781 cccgctaaga acctcaagat gcccggcgtc tactatgtgg accacagact ggaaagaatc
      841 aaggaggccg acaaagagac ctacgtcgag cagcacgagg tggctgtggc cagatactgc
      901 gacctcccta gcaaactggg gcacaaactt aatggcatgg acgagctgta caagtaaacc
      961 cagctttctt gtacaaagtg gtgattcgag gccgcgactc tagaattcga tatcaagctt
     1021 atcgataatc aacctctgga ttacaa
//
