LOCUS       sequence-132857-         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses RGVG-M6 T7 RNA polymerase variant with enhanced 
            transcription of 2' modified RNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Meyer AJ, Garry DJ, Hall B, Byrom MM, McDonald HG, Yang X, Yin YW, 
            Ellington AD
  TITLE     Transcription yield of fully 2'-modified RNA can be increased by the
            addition of thermostabilizing mutations to T7 RNA polymerase 
            mutants.
  JOURNAL   Nucleic Acids Res. 2015 Sep 3;43(15):7480-8. doi: 
            10.1093/nar/gkv734. Epub 2015 Jul 24.
  PUBMED    26209133
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkv734"; journalName: "Nucleic Acids Res"; date: 
            "2015-09-3- 3"; volume: "43"; issue: "15"; pages: "7480-8"
COMMENT     Sequence Label: pQE-WT-RGVG-M6
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3..20
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cgcatcacca tcaccatcac ggatccaaca cgattaacat cgctaagaac gacttctctg
       61 acatcgaact ggctgctatc ccgttcaaca ctctggctga ccattacggt gagcgtttag
      121 ctcgcgaaca gttggccctt gagcatgagt cttacgagat gggtgaagca cgcttccgca
      181 agatgtttga gcgtcaactt aaagctggtg aggttgcgga taacgctgcc gccaagcctc
      241 tcatcactac cctactccct aagatgattg cacgcatcaa cgactggttt gaggaagtga
      301 aagctaagcg cggcaagcgc ccgacagcct tccagttcct gcaagaaatc aagccggaag
      361 ccgtagcgta catcaccatt aagaccactc tggcttgcct aaccagtgct gacaatacaa
      421 ccgttcaggc tgtagcaagc gcaatcggtc gggccattga ggacgaggct cgcttcggtc
      481 gtatccgtga ccttgaagct aagcacttca agaaaaacgt tgaggaacaa ctcaacaagc
      541 gcgtagggca cgtctacaag aaagcattta tgcaagttgt cgaggctgac atgctctcta
      601 agggtctact cggtggcgag gcgtggtctt cgtggcataa ggaagactct attcatgtag
      661 gagtacgctg catcgagatg ctcattgagt caaccggaat ggttagctta caccgccaaa
      721 atgctggcgt agtaggtcaa gactctgaga ctatcgaact cgcacctgaa tacgctgagg
      781 ctatcgcaac ccgtgcaggt gcgctggctg gcatctctct gatgttcca
//
