LOCUS       sequence-132897-         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Enslen H, Raingeaud J, Davis RJ
  TITLE     Selective activation of p38 mitogen-activated protein (MAP) kinase 
            isoforms by the MAP kinase kinases MKK3 and MKK6.
  JOURNAL   J Biol Chem. 1998 Jan 16. 273(3):1741-8.
  PUBMED    9430721
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1998 Jan 16. 273(3):1741-8."
COMMENT     Sequence Label: pCDNA3 Flag p38 alpha
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             95..118
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctt cggcagccaa catggactat aaggacgatg atgacaaaat
      121 gtcgcaggag aggcccacgt tctaccggca ggagctgaac aagaccatct gggaggtgcc
      181 cgaacgatac cagaacctgt ccccggtggg ctcgggcgcc tatggctcgg tgtgtgctgc
      241 ttttgataca aagacggggc atcgtgtggc agttaagaag ctgtcgagac cgtttcagtc
      301 catcattcac gccaaaagga cctaccgaga gttgcgtctg ctgaagcaca tgaaacacga
      361 aaatgtgatt ggtctgttgg atgtgttcac acccgcaagg tcactggagg aattcaatga
      421 cgtgtacctg gtgacccatc tcatgggggc ggacctgaac aacatcgtga agtgccagaa
      481 gctgaccgac gaccacgttc agtttctcat ctaccagatc ctccgagggc tgaagtatat
      541 acattcggct gacataattc acagggacct aaagcccagc aacctagctg tgaacgaaga
      601 ctgtgagctc aagattctgg attttgggct ggctcggcac actgatgatg agatgacagg
      661 ctacgtggct accaggtggt accgagcccc agagatcatg ctgaattgga tgcactataa
      721 ccagacagtg gatatttggt ccgtgggctg catcatggct gagctgttga ccggaagaac
      781 gttgtttcct ggtacagacc atattgatca gttgaagctc att
//
