LOCUS       sequence-132961-        1058 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses FLAG tagged human BRD4 ET domain (608-699 aa).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Shen C, Ipsaro JJ, Shi J, Milazzo JP, Wang E, Roe JS, Suzuki Y, 
            Pappin DJ, Joshua-Tor L, Vakoc CR
  TITLE     NSD3-Short Is an Adaptor Protein that Couples BRD4 to the CHD8 
            Chromatin Remodeler.
  JOURNAL   Mol Cell. 2015 Nov 24. pii: S1097-2765(15)00824-2. doi: 
            10.1016/j.molcel.2015.10.033.
  PUBMED    26626481
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2015 Nov 24. pii: S1097-2765(15)00824-2. doi: 
            10.1016/j.molcel.2015.10.033."
COMMENT     Sequence Label: pcDNA-ET
FEATURES             Location/Qualifiers
     source          1..1058
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             29..43
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     promoter        complement(353..371)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(354..371)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(391..408)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    397..621
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(754..773)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cgagctcggn tctaccatgg nctacaagga cgacgatgac aagaagccta tgtcctatga
       61 ggagaagcgg cagctcagct tggacatcaa caagctcccc ggcgagaagc tgggccgcgt
      121 ggtgcacatc atccagtcac gggagccctc cctgaagaat tccaaccccg acgagattga
      181 aatcgacttt gagaccctga agccgtccac actgcgtgag ctggagcgct atgtcacctc
      241 ctgtttgcgg aagaaaagga aacctcaagc tgagaaagtt gatgtgattg ccggctcctc
      301 caagatgaag ggcttctcgt aataactcga gcatgcatct agagggccct attctatagt
      361 gtcacctaaa tgctagagct cgctgatcag cctcgactgt gccttctagt tgccagccat
      421 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
      481 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
      541 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
      601 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
      661 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
      721 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
      781 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
      841 gatttagtgc tttacggcac ctcgacccca aaaancttga ttagggtgat ggttcacgta
      901 nngggccatc gccctgatag acggttttnc nccntttgac gttgganncc acgttcttta
      961 atantggact cttgttccaa actggaannn nncncaaccn tatctcggtc tattctttng
     1021 attanaaggn nttnnncnna ttncggnnnn nnntnnaa
//
