LOCUS       sequence-132965-        1280 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses FLAG tagged human BRD4 ET domain (608-699 aa) with I654Q 
            mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Shen C, Ipsaro JJ, Shi J, Milazzo JP, Wang E, Roe JS, Suzuki Y, 
            Pappin DJ, Joshua-Tor L, Vakoc CR
  TITLE     NSD3-Short Is an Adaptor Protein that Couples BRD4 to the CHD8 
            Chromatin Remodeler.
  JOURNAL   Mol Cell. 2015 Nov 24. pii: S1097-2765(15)00824-2. doi: 
            10.1016/j.molcel.2015.10.033.
  PUBMED    26626481
REFERENCE   2  (bases 1 to 1280)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2015 Nov 24. pii: S1097-2765(15)00824-2. doi: 
            10.1016/j.molcel.2015.10.033."
COMMENT     Sequence Label: pcDNA-ET-I654Q
FEATURES             Location/Qualifiers
     source          1..1280
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             28..42
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     promoter        complement(352..370)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(353..370)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(390..407)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    396..620
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      666..1020
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(753..772)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     gap             1148..1165
                     /estimated_length=18
     gap             1199..1215
                     /estimated_length=17
     gap             1241..1251
                     /estimated_length=11
     gap             1253..1265
                     /estimated_length=13
ORIGIN
        1 gagctcggnt ctaccatggn ctacaaggac gacgatgaca agaagcctat gtcctatgag
       61 gagaagcggc agctcagctt ggacatcaac aagctccccg gcgagaagct gggccgcgtg
      121 gtgcacatca tccagtcacg ggagccctcc ctgaagaatt ccaaccccga cgagattgaa
      181 caggactttg agaccctgaa gccgtccaca ctgcgtgagc tggagcgcta tgtcacctcc
      241 tgtttgcgga agaaaaggaa acctcaagct gagaaagttg atgtgattgc cggctcctcc
      301 aagatgaagg gcttctcgta ataactcgag catgcatcta gagggcccta ttctatagtg
      361 tcacctaaat gctagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
      421 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
      481 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
      541 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
      601 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
      661 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
      721 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
      781 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
      841 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
      901 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
      961 tagtggactc ttgttccaac tggaacacac tcaccntatc tcggntattc ttttgattna
     1021 angggatttt gccgattnng nntattggtt aaaaatgagc tgattnacaa aattnnnncn
     1081 nntaatnnng ggnanngggg nnngttaggn nggnaaancc cnngnnccnn nnnngncaaa
     1141 nttnnaannn nnnnnnnnnn nnnnncnnnn gnngnnaann ncnnnnncnn nngggnaann
     1201 nnnnnnnnnn nnnnnttnnc nnnnanttcc cccccaannc nnnnnnnnnn ncnnnnnnnn
     1261 nnnnnccccc ngnnnnnttt
//
