LOCUS       sequence-133069-         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Multisite gateway entry clone for adding C-terminal fusions of human
            2PH-PLC-delta.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Ariotti N, Hall TE, Rae J, Ferguson C, McMahon KA, Martel N, Webb 
            RE, Webb RI, Teasdale RD, Parton RG
  TITLE     Modular Detection of GFP-Labeled Proteins for Rapid Screening by 
            Electron Microscopy in Cells and Organisms.
  JOURNAL   Dev Cell. 2015 Nov 23;35(4):513-25. doi: 
            10.1016/j.devcel.2015.10.016. Epub 2015 Nov 12.
  PUBMED    26585296
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2015.10.016"; journalName: "Dev Cell"; date: 
            "2015-11-23- 23"; volume: "35"; issue: "4"; pages: "513-25"
COMMENT     Sequence Label: pME-2PH-PLCd
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    59..83
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      87..96
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttgactgata gtgacctgtt cgttgcaaca aattgatgag caatgctttt ttataatgcc
       61 aactttgtac aaaaaagcag gctgccgcca ccatggactc gggccgggac ttcctgaccc
      121 tgcacggcct acaggatgat gaggatctac aggcgctgct gaagggcagc cagctcctga
      181 aggtgaagtc cagctcatgg aggagagagc gcttctacaa gttgcaggag gactgcaaga
      241 ccatctggca ggagtcccgc aaggtcatgc ggaccccgga gtcccagctg ttctccatcg
      301 aggacattca ggaggtgcga atggggcacc gcacggaggg tctggagaag ttcgcccgtg
      361 atgtgcccga ggaccgctgc ttctccattg tcttcaagga ccagcgcaat acactagacc
      421 tcatcgcccc atcgccagct gatgcccagc actgggtgct ggggctgcac aaaattatcc
      481 atcacagcgg atctatggac cagcgtcaga agctacagca ctggattcac tcctgcttgc
      541 gaaaagctga caaaaacaag gacaacaaga taggcggcag cggcggcagc ggcggcgata
      601 gcggtagaga ttttctgact ctgcatggac tgcaggacga cgaggacctg caggccctgc
      661 tgaagggcag ccagctgctg aaggtgaaga gcagcagctg gcgccgcgag cgcttctaca
      721 agctgcagga ggactgcaag accatctggc aggagagccg caaggtgatg cgcacccccg
      781 agagccagct gttcagcatc gaggacatcc agga
//
