LOCUS       sequence-133080-         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of eIF4A1 P159E mutant construct in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Wolfe AL, Singh K, Zhong Y, Drewe P, Rajasekhar VK, Sanghvi VR, 
            Mavrakis KJ, Jiang M, Roderick JE, Van der Meulen J, Schatz JH, 
            Rodrigo CM, Zhao C, Rondou P, de Stanchina E, Teruya-Feldstein J, 
            Kelliher MA, Speleman F, Porco JA Jr, Pelletier J, Ratsch G, Wendel 
            HG
  TITLE     RNA G-quadruplexes cause eIF4A-dependent oncogene translation in 
            cancer.
  JOURNAL   Nature. 2014 Sep 4;513(7516):65-70. doi: 10.1038/nature13485. Epub 
            2014 Jul 27.
  PUBMED    25079319
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature13485"; journalName: "Nature"; date: "2014-09-4- 4"; 
            volume: "513"; issue: "7516"; pages: "65-70"
COMMENT     Sequence Label: MIG-eIF4A1 P159E
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     45..64
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        45..63
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             95..117
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     regulatory      126..135
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             138..164
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 actccttctc taggcgccgg aattagatct cgatcccgcg aaattaatac gactcactat
       61 agggagacca caacggtttc cctctagaaa taattttgtt taactttaag aaggagatat
      121 acatagccac catggcttac ccatacgacg tcccagacta cgctatgtct gcgagtcagg
      181 attctcgatc cagagacaat ggccccgacg ggatggagcc ggaaggcgtc atcgagagta
      241 actggaacga gattgtggat agctttgatg acatgaatct ctcagagtcc ctcctccgtg
      301 gtatttatgc ctatggtttt gagaagccct ctgccatcca gcagcgagct attcttcctt
      361 gtatcaaggg ttatgatgtg attgctcaag cccagtctgg gactgggaaa acagctacat
      421 ttgccatatc aattctgcag cagattgaat tagatctaaa ggccactcag gctttggttc
      481 tggcacccac acgtgaattg gctcagcaga tacaaaaggt ggttatggca ttaggagact
      541 acatgggtgc ctcttgtcat gcctgcattg ggggcaccaa tgtgcgtgct gaggtgcaga
      601 agctgcagat ggaagctccc catatcatcg tgggtaccga aggccgggtg tttgacatgc
      661 ttaaccggag atacctgtcc cccaaataca tcaagatgtt cgtactggat gaagcagatg
      721 aaatgttaag ccgagggttc aaggatcaga tctatgacat attccagaag ctcaacagca
      781 acacacaggt agtt
//
