LOCUS       sequence-133081-         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of eIF4A1 F163L mutant in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Wolfe AL, Singh K, Zhong Y, Drewe P, Rajasekhar VK, Sanghvi VR, 
            Mavrakis KJ, Jiang M, Roderick JE, Van der Meulen J, Schatz JH, 
            Rodrigo CM, Zhao C, Rondou P, de Stanchina E, Teruya-Feldstein J, 
            Kelliher MA, Speleman F, Porco JA Jr, Pelletier J, Ratsch G, Wendel 
            HG
  TITLE     RNA G-quadruplexes cause eIF4A-dependent oncogene translation in 
            cancer.
  JOURNAL   Nature. 2014 Sep 4;513(7516):65-70. doi: 10.1038/nature13485. Epub 
            2014 Jul 27.
  PUBMED    25079319
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature13485"; journalName: "Nature"; date: "2014-09-4- 4"; 
            volume: "513"; issue: "7516"; pages: "65-70"
COMMENT     Sequence Label: MIG-eIF4A1 F163L
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     44..63
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        44..62
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             94..116
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     regulatory      125..134
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             137..163
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ctccttctct aggcgccgga attagatctc gatcccgcga aattaatacg actcactata
       61 gggagaccac aacggtttcc ctctagaaat aattttgttt aactttaaga aggagatata
      121 catagccacc atggcttacc catacgacgt cccagactac gctatgtctg cgagtcagga
      181 ttctcgatcc agagacaatg gccccgacgg gatggagccg gaaggcgtca tcgagagtaa
      241 ctggaacgag attgtggata gctttgatga catgaatctc tcagagtccc tcctccgtgg
      301 tatttatgcc tatggttttg agaagccctc tgccatccag cagcgagcta ttcttccttg
      361 tatcaagggt tatgatgtga ttgctcaagc ccagtctggg actgggaaaa cagctacatt
      421 tgccatatca attctgcagc agattgaatt agatctaaag gccactcagg ctttggttct
      481 ggcacccaca cgtgaattgg ctcagcagat acaaaaggtg gttatggcat taggagacta
      541 catgggtgcc tcttgtcatg cctgcattgg gggcaccaat gtgcgtgctg aggtgcagaa
      601 gctgcagatg gaagctcccc atatcatcgt gggtacccct ggccgggtgt tggacatgct
      661 taaccggaga tacctgtccc ccaaatacat caagatgttc gtactggatg aagcagatga
      721 aatgttaagc cgagggttca aggatcagat ctatgacata ttccagaagc tcaacagcaa
      781 cacacaggta gttttgttgt ctgctacaat gccttctgat gtccttgagg tgaccaagaa
//
