LOCUS       sequence-133219-         669 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Singlet donor (phase 0) for in vivo RMCE with MiMIC inserts to make 
            Gal4 driver..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Diao F, Ironfield H, Luan H, Diao F, Shropshire WC, Ewer J, Marr E, 
            Potter CJ, Landgraf M, White BH
  TITLE     Plug-and-play genetic access to drosophila cell types using 
            exchangeable exon cassettes.
  JOURNAL   Cell Rep. 2015 Mar 3;10(8):1410-21. doi: 
            10.1016/j.celrep.2015.01.059. Epub 2015 Feb 26.
  PUBMED    25732830
REFERENCE   2  (bases 1 to 669)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.01.059"; journalName: "Cell Rep"; date: 
            "2015-03-3- 3"; volume: "10"; issue: "8"; pages: "1410-21"
COMMENT     Sequence Label: pC-(loxP2-attB2-SA(0)-T2A-Gal4-Hsp70)
FEATURES             Location/Qualifiers
     source          1..669
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     181..199
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    complement(231..264)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    380..449
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 gaaggaggca aacagcgctg actttgagtg gaatgtcatt ttgagtgaga ggtaatcgaa
       61 agaacctggt acatcaaata cccttggatc gaagtaaatt taaaactgat cagataagtt
      121 caatgatatc cagtgcagta aaaaaaaaaa atgttttttt tatctacttt ccgcaaaaat
      181 gggttttatt aacttacata catactagaa ttaggccttc tagtggatct ataacttcgt
      241 atagcataca ttatacgaag ttattcaaat ttaaataata acaattataa tttttactct
      301 ggcataattg tatgcaattt gcaaatgcag cttcgttttt taggggtgtg aatatttatt
      361 gtatgatgta ggtcacggtc tcgaagccgc ggtgcgggtg ccagggcgtg cccttgggct
      421 ccccgggcgc gtactccacc tcacccatct ggtccatcat gatggtcgac aagcttagaa
      481 gttcaaatgg gctttcatat ttactttttt ctctgagtgg gtaaggtcat tgtggggtct
      541 taagggctaa agggtagtga tgatgaaggc atttgatgcc attgttcaat aacttacctc
      601 gccggaacct ccgcttccac cgctacctcc ggatccaaac gagtttttaa gcaaactcac
      661 tccctgaca
//
