LOCUS       sequence-133220-         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Singlet donor (phase 0) for in vivo RMCE with MiMIC inserts to make 
            Gal4 driver..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Diao F, Ironfield H, Luan H, Diao F, Shropshire WC, Ewer J, Marr E, 
            Potter CJ, Landgraf M, White BH
  TITLE     Plug-and-play genetic access to drosophila cell types using 
            exchangeable exon cassettes.
  JOURNAL   Cell Rep. 2015 Mar 3;10(8):1410-21. doi: 
            10.1016/j.celrep.2015.01.059. Epub 2015 Feb 26.
  PUBMED    25732830
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.01.059"; journalName: "Cell Rep"; date: 
            "2015-03-3- 3"; volume: "10"; issue: "8"; pages: "1410-21"
COMMENT     Sequence Label: pC-(loxP2-attB2-SA(0)-T2A-Gal4-Hsp70)
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    147..216
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     CDS             383..436
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 acttcgtata atgtatgcta tacgaagtta tcaattggct tattgggatt tcaatttaag
       61 atgctgctaa ataaagttag tcacttacgg actggggagg caccccgtca tcactcgcca
      121 gaaatgcaat tgatgtaggt cacggtctcg aagccgcggt gcgggtgcca gggcgtgccc
      181 ttgggctccc cgggcgcgta ctccacctca cccatctggt ccatcatgat ggtcgactct
      241 agaagtcgat ccaacatggc gacttgtccc atccccggca tgtttaaata tactaattat
      301 tcttgaacta attttaatca accgatttat ctctcttccg caggtgggag gttccggtgg
      361 aagcggaggt agcggcggat ccgagggccg cggcagcctg ctgacctgcg gcgatgtgga
      421 ggagaacccc gggcccatga agctgctgag cagcatcgag caggcctgcg atatctgccg
      481 cctgaagaag ctgaagtgca gcaaggagaa gcccaagtgc gccaagtgcc tgaagaacaa
      541 ctgggagtgc cgctacagcc ccaagaccaa gcgcagcccc ctgacccgcg cccacctgac
      601 cgaggtggag agccgcctgg agcgcctgga gcagctgttc ctgctgatct tcccccgcga
      661 ggatctggat atgatcctga agatggatag cctgcaggat atcaaggccc tgctgaccgg
      721 cctgttcgtg caggataacg tgaacaagga tgccgtgacc gatcgcctgg ccagcgtgga
      781 gaccgatatg cccctgaccc tgcgccagca ccgcatcagc g
//
