LOCUS       sequence-133241-       10482 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  It encodes a fluorescent protein that can be fused to a gene of 
            interest using SEC CRISPR strategy.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10482)
  TITLE     Hobert plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10482)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pUA97
FEATURES             Location/Qualifiers
     source          1..10482
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             661..966
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             1908..1928
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     protein_bind    complement(1946..1979)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             1989..2018
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      complement(3835..4158)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    6076..6100
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6103..7128
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6103..7128
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(7129..7153)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7506..7539)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             7558..7581
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             7585..7608
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             7612..7635
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(7683..7988)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPG*RMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(8261..8277)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8261..8277)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8274..8296)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8285..8301
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8309..8315,8316..8333,8334..8339))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8354..8375
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8492..8509)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8663..9251)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8743..8762)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9422..10213,10214..10282))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10045..10064
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10283..10387)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10455..10473
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtcgccg gcactagtta cccgaagtat
      421 gtcaaaaaga ggtatgctat gaagcagcgt attacagtga cagttgacag cgacagctat
      481 cagttgctca aggcatatat gatgtcaata tctccggttc ggtaagcaca accatgcaga
      541 atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg
      601 aggtcgcccg gtttattgaa atgaacggct cttttgccga cgagaacagg ggctggtgaa
      661 atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta
      721 cagagtgata ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt
      781 ctgctgtcag ataaagtccc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc
      841 tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg
      901 gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga
      961 atataaatgt caggctccct tatacacagc cagtctgcag gtcgacctag ggagcatcgg
     1021 gagcctcagg agcatcggga gcctcaggag catcgatgtc agagcttatt aaggagaata
     1081 tgcatatgaa actgtacatg gaaggaaccg tggacaacca tcactttaag tgcacttcgg
     1141 agggggaagg caaaccgtac gagggcaccc agaccatgcg aatcaaggtg gtcgagggag
     1201 gcccactgcc cttcgccttc gacattctgg taagtttaaa catatatata ctaactaacc
     1261 ctgattattt aaattttcag gctactagct tcctgtacgg cagcaagact tttattaatc
     1321 acacccaggg catcccggac ttcttcaaac agtcgttccc tgagggcttc acatgggagc
     1381 gtgtgaccac atacgaagac ggtggcgttc tcaccgctac ccaggacacc agcctccagg
     1441 acggttgcct catatataat gtggtaagtt taaacagttc ggtactaact aaccatacat
     1501 atttaaattt tcagaagatc cggggagtaa acttcacatc caatggtccg gttatgcaga
     1561 agaaaacact cggctgggag gccttcaccg agacgctgta tccggctgac ggaggcctgg
     1621 aaggtcgaaa cgacatggcc ctgaagctcg tcggcggtat ccatctcatc gcaaacgcta
     1681 agaccacata tagatccaag aaaccggtaa gtttaaacat gattttacta actaactaat
     1741 ctgatttaaa ttttcaggct aagaacctta aaatgccggg agtctactat gttgactaca
     1801 gactggaacg gatcaaggag gccaacaacg agacctacgt cgagcagcac gaggttgcag
     1861 ttgccagata ctgcgacctc ccgagcaaac tgggtcacaa gcttaatgag aatctgtact
     1921 ttcaatccgg aaaggtaagt ttaaaataac ttcgtatagc atacattata caaagttatt
     1981 ttcagggaga gcaaaagctt atctccgagg aggacctcta aggatgatcg acgccaacgt
     2041 cgttgaattt tcaaatttta aatactgaat atttgttttt tttcctatta tttatttatt
     2101 ctctttgtgt tttttttctt gctttctaaa aaattaattc aatccaaatc taaacatttt
     2161 tttttctctt tccgtctccc aattcgtatt ccgctcctct catctgaaca caatgtgcaa
     2221 gtttatttat cttctcgctt tcatttcatt aggacgtggg gggaattggt ggaaggggga
     2281 aacacacaaa aggatgatgg aaatgaaata aggacacaca atatgcaaca acattcaatt
     2341 cagaaatatg gaggaaggtt taaaagaaaa cataaaaata tatagaggag gaaggaaact
     2401 agctgccctc gattgtctat cttccattct caccaagttt caacaacttt attcagtcat
     2461 tcttctctgg ttcatttccc aaccaatctt cccgaataaa gcccatctgt cctttcggcc
     2521 acattcctca cctacatccg tactccttat ctcccgttct tatccagtct tttatcgtct
     2581 ctgcgtctcg cacactgtct cgtcggtaat gtaaaaaaaa aacaaaaaaa acttgaaaac
     2641 ttgcggaaag atttcctaat aaaaggagac gaatccggta ggaaatcgac aagtctctgc
     2701 ttctcgaaga ctaggtgagt tacatttctt tggccgaatt attttgaaat cctaaatttg
     2761 ataatttcag agatgtctgt aaaacttgcg tgttatgtga cggcttcggt cactgtcgcc
     2821 actcttatgg tatgcttcat gaccatgtca accatctact cggaggttga tggattcaga
     2881 gagaagcttg ataccgagat gaatgtgttc agagtgagtt aaattatatt ttgaatttta
     2941 aataattttt aattttctag caatctacca atggattgtg gaaggacata gttgtcatcg
     3001 gaagatctag caagcgtgtc cgttgtcaat atgaagagac caacgctacc ccaactccac
     3061 atgctgatgg atccccatct gctccaccag gtcaaccacc agcagttcca ccagtcttca
     3121 accagccaaa gactccaaat ggagccaatg gaaatggacc aacctgcaac tgcaatgctg
     3181 ataacaagtg cccagctgga ccatccggac caaagggagt tccaggagtt ccaggactcg
     3241 acggagttcc aggacttgac ggtgttccag gagttggagc tgatgatatc gctccacaac
     3301 gcgagtctgt cggatgcttc acttgcccac aaggaccagt tggaccacca ggagctcttg
     3361 gaagaccagg accacgtgga cttccaggac caagaggaca aaatggaaac ccaggaagag
     3421 atggacaacc aggacatcca ggagagcaag gatcatccgg tcaaatcgga aagatcggag
     3481 agccaggacc accaggagag aagggacgcg acgccgagca tccaatcgga agaccaggac
     3541 caaagggacc aagaggagat caaggaccaa caggaccagc tggacagaac ggtcttcacg
     3601 gaccaccagg agagccagga accgttggac cagaaggacc atctggaaag caaggacgtc
     3661 aaggaccaga cggaacccag ggagagactg gaccagacgg aagaccagga aaggatgccg
     3721 agtactgcca gtgcccagac aagtctccac catcagaggc tgtcaacgcc aaccgtggat
     3781 acagaaatat ctaaattgtt ggtgttttct aataaaatat ttgagattca gtgagacttt
     3841 tttcttggcg gcacaataaa gtttcctctt tttgttgggt cactcaacga acacaataca
     3901 aatatttgag cgctcgcatt tttctcaacc tttcgggttt aaagcacttc aaaatggaat
     3961 tgaagagaga ttgtgaaagt acatcagaca gaatcacatt ttgatagcat tcacttcact
     4021 cagatgcaaa cttttcagtg actataaaaa gtcataaaat aataacatta aaaaagcaaa
     4081 taaattagcg agagaatttt ttgacaaaaa gaaagaagag tgatagagaa gaagggaatg
     4141 cttgaaagga ttttgcattt atcttaatca ccgtcttcga gaagacgaac catggcgcca
     4201 gtttcagaat ccaagtttct aatatagttc atcacaatgt tgacgttggt ccaacctccg
     4261 gcttgcataa tctctgggat ggaaactcca gcgcgagcca tgtcacgggc agctccgaca
     4321 cgggcggaat gtccagacca agccaagtaa cgttgtccgg aatcatcctt ggctccgtag
     4381 atgagacggt gggtagcttc gaagattcct gaaaatttat tttcagctta ttcttagcta
     4441 atatgttgtt taaacttacc ttcaagagct cgagtcgaga gttgtgatgt tgcactcggg
     4501 gcagcaactc cgttcttgcg aacacgacag aacaggtagt tgtttggatc atcggcgact
     4561 ccggaaaccg aaatccagcg ctcaacgagc ttggtaactc caagggagag cgctttttcg
     4621 actccggcag tcgagaccag cgtttttgta cgtccgatgt ggatgagcat tcttcctccg
     4681 tctgtgcgac taatatcctt gacgcgaatg cgggcaattt cggcgatacg gagaagtgtg
     4741 ttgtaagcaa ttccgaggaa ggcgaggttt cggatgtcct ggcaacgatc ggaattttcc
     4801 ataagagaac ggacctgatc gaagtcggtt ctgaaaattt cgtcaagtct taggattgaa
     4861 tacattaata tttaaactta cctttcgaag gcgagggctt gcttggctct ctctccagca
     4921 tcaacatttt ccttgcggat acgtctcatt acgagagata cggcattaga atctgatggg
     4981 cgtggaagtc cagatcggcg atggagcata ttgagctgtc cgagatgttg ttggatcgtc
     5041 ttcaccgcca atccacgagc ttggaggtag agcaagtagt cacgaacgtc ttctggctcg
     5101 gctgggaacc acttgcggtt gttaagcttg caccaggcag cccaagaacg gcacactgag
     5161 agaagcatct tccaggtgtg ctcggagaag gcttgtctgt cacggaacat atccatgagg
     5221 ttcttgcgaa cttcatcact tgtcgcgtca actggaagtg ctggaagatt ttgatgaaca
     5281 gtgagaagat ttgacatatt ttcgaagttt tttagatgca ctagaacaaa gcgtgttggc
     5341 ttcctctgag cccgctttcc ttatataccc gcattctgca gccttacaga atgttctaga
     5401 aggtcctaga tgcattcgtt tgaaaatact cccggtgggt gcaaagagac gcagacggaa
     5461 aatgtatctg ggtctcttta ttgtgtacac tacttttcca tgtaccgaat gtgagtcgcc
     5521 ctccttttgc aacaagcagc tcgaatgttc tagaaaaagg tggaaaatag tataaatacc
     5581 gttgaaaata aataccgaac aacatttgct ctaattgtga aattagaaat cttcaaacta
     5641 taatcatgtc actttaccac tatttccgtc cagctcaacg ttccgttttt ggtgatcatt
     5701 tttgctttcg tcgtaaatct acacacgcgt ctcttccgtg cgagagtcca agccagcagc
     5761 caaattcgtt gactgagtat tcaacgttta tacgttgtcg gcaacgagaa ataggaaaat
     5821 gcatcgggaa atgttctttt ttcgattttt tccaaggttt tgacaaattt taccacgaat
     5881 tttgctatgt tttcaattaa aaaatatgtt attcaactgt ttctatgagg aaaataaggc
     5941 tttgcatgta attttcttat tcagcataat ttttaattaa tttgaatttt ctgtcctaac
     6001 gtttattttg ttttcttggt tatgactgat ctgaaattaa tttttgaatt ttaaggtaat
     6061 atgtcaggcg gtgccgcaag tttgtacaaa aaagcaggct ccatgaaaaa gcctgaactc
     6121 accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg
     6181 cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat
     6241 gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac
     6301 tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggaatt cagcgagagc
     6361 ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc
     6421 gaactgcccg ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat
     6481 cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca
     6541 tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg
     6601 gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag
     6661 gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg
     6721 gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa
     6781 tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg
     6841 cgctacttcg agcggaggca tccggagctt gcaggatcgc cgcggctccg ggcgtatatg
     6901 ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca
     6961 gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt
     7021 acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc
     7081 gatagtggaa accgacgccc cagcactcgt ccgagggcaa aggaatagac ccagctttct
     7141 tgtacaaagt gggtccaatt actcttcaac atccctacat gctctttctc cctgtgctcc
     7201 caccccctat ttttgttatt atcaaaaaac ttctcttaat ttctttgttt tttagcttct
     7261 tttaagtcac ctctaacaat gaaattgtgt agattcaaaa atagaattaa ttcgtaataa
     7321 aaagtcgaaa aaaattgtgc tccctccccc cattaataat aattctatcc caaaatctac
     7381 acaatgttct gtgtacactt cttatgtttt ttacttctga taaatttttt tgaaacatca
     7441 tagaaaaaac cgcacacaaa ataccttatc atatgttacg tttcagttta tgaccgcaat
     7501 ttttaataac ttcgtatagc atacattata cgaagttatt ttcagggagc cggatctgat
     7561 tataaagacg atgacgataa gcgtgactac aaggacgacg acgacaagcg tgattacaag
     7621 gatgacgatg acaagagact agttcgacct gcagactggc tgtgtataag ggagcctgac
     7681 atttatattc cccagaacat caggttaatg gcgtttttga tgtcattttc gcggtggctg
     7741 agatcagcca cttcttcccc gataacggag accggcacac tggccatatc ggtggtcatc
     7801 atgcgccagc tttcatcccc gatatgcacc accgggtaaa gttcacgggg gactttatct
     7861 gacagcagac gtgcactggc cagggggatc accatccgtc acccgggcgt gtcaataata
     7921 tcactctgta catccacaaa cagacgataa cggctctctc ttttataggt gtaaacctta
     7981 aactgcattt caccagcccc tgttctcgtc ggcaaaagag ccgttcattt caataaaccg
     8041 ggcgacctca gccatccctt cctgattttc cgctttccag cgttcggcac gcagacgacg
     8101 ggcttcattc tgcatggttg tgcttaccga accggagata ttgacatcat atatgccttg
     8161 agcaactgat agctgtcgct gtcaactgtc actgtaatac gctgcttcat agcatacctc
     8221 tttttgacat acttcgggtt cctaggaaat cgataacatg gtcatagctg tttcctgtgt
     8281 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     8341 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     8401 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     8461 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     8521 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     8581 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     8641 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     8701 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     8761 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     8821 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     8881 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     8941 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     9001 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     9061 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     9121 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     9181 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     9241 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     9301 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     9361 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     9421 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     9481 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     9541 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     9601 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     9661 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     9721 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     9781 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     9841 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     9901 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     9961 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
    10021 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
    10081 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
    10141 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
    10201 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
    10261 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
    10321 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
    10381 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
    10441 cattaaccta taaaaatagg cgtatcacga ggccctttcg tc
//
