LOCUS       sequence-133274-         944 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  (control) when used to produce lentivirus, express physiological 
            levels of insert .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2014-12-23"; volume: "7(357):ra121. doi"; pages: " 
            10.1126/scisignal.aaa1877"
COMMENT     Sequence Label: MEK1-pcw107-V5
FEATURES             Location/Qualifiers
     source          1..944
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(48..89)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    97..121
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 attttgtaat ccagaggttg attgtcgact taacgcggtc attactacgt agaatcgaga
       61 ccgaggagag ggttagggat aggcttacca ccggtaacca ctttgtacaa gaaagttggc
      121 aagacgccag cagcatgggt tggtgtgctg ggctggttaa ggccgatggt ggagcagagc
      181 caacctgcaa aatccacttc ctcagcatca gatctcttga taaaagcatg aaccatgagt
      241 tgcttcaaat ctgctctctc tgcggggttt tttattaagc atttattcac aaaatcttga
      301 aattccagac tgaacactcc actgggcagt tttggaggag gctcgttgac tatgtaatcc
      361 aacaactcaa aaattgccat gggaggtcgg ctgtccattc catatgagct aaggggcctc
      421 ccgggggtcc ttggcctggg tggggtctca gccgcatctc cttccacctg gcacccaaac
      481 atcagctcca gctccttggc atctggagga gggatgggat acctcccaac cgccatctct
      541 accagagaca gtcccatgct ccagatgtct gactgcacag agtaatgagt cccctggagt
      601 ctttctggcg acatgtagga ccttgtgccc acgaaggagt tggccatgga gtcgatgagc
      661 tgcccgctga ccccaaagtc acagagcttg atctccccac gggagttgac taggatgttg
      721 gagggcttga catctctgtg catgatcttg tgcttctcca tcagatatgt caggcctttt
      781 attacagcaa tgctaacttt tcctaaaatt tgttcaggaa ttcttccagc tttcttcagg
      841 acttgatcca gagaacctcc atccatgtgc tccatgcaga tactgatctc gccatcgctg
      901 tagaacgcac catagaagcc cacgatgtac ggagagttgc actc
//
