LOCUS       sequence-133293-         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  reporter with qacR 2 on single plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   de Los Santos EL, Meyerowitz JT, Mayo SL, Murray RM
  TITLE     Engineering Transcriptional Regulator Effector Specificity Using 
            Computational Design and In Vitro Rapid Prototyping: Developing a 
            Vanillin Sensor.
  JOURNAL   ACS Synth Biol. 2015 Aug 19.
  PUBMED    26262913
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Aug 19."
COMMENT     Sequence Label: pES006 (pTet-qacR 2/pQacA-deGFP)
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    121..139
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             183..194
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             775..792
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tcatcgataa gcttccgatg gcgcgccgag aggctttaca ctttatgctt ccggctggtc
       61 tcagcatcca cgcattataa cgtcgtatta gctgcctttc gtcttcaata attcttgaca
      121 tccctatcag tgatagagat acttcgtgca atttttatat caccgccagg ggtacaacaa
      181 gcaaagagga gaaatactag catgaacttg aaagataaga ttttgggtgt tgctaaagag
      241 ctgtttatca aaaacggtta caatgctact accactggtg agattgtgaa attgtctgaa
      301 tcttctaaag ggaatctata ctatcatttc aaaacgaagg aaaatttgtt cttggaaatc
      361 ttgaacattc aactgagtaa atatcaagaa caatggaaaa aggagcagat taaagctaaa
      421 actaatagag aaaagtttta tctgtataat gagttgtctt tgactacaca atattactat
      481 ccactacaga acgcacagat cgagttctac actgaatact ataagactaa ctccatcaat
      541 gaaaaacaga acaaatacca aaataagtat atcgatgcat atcacgttat tttcaaagaa
      601 ggtaatctta acggtgagtg gagtataaat gatgttaatg ccgtttctaa aattgctgct
      661 atggctgttt taggtattgt tatgttcaca catgagcaaa acatcaacga aagaattaag
      721 ttgatgaaca agttctctca gatctttttg aatggtctta gtaaaggttc tggtcaccat
      781 catcaccacc attaatgaac caggcatcaa ataaaacgaa aggctcagtc gaaagactg
//
