LOCUS       sequence-133294-         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tn7-based lux reporter for promoter cloning in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Glassing A, Lewis TA
  TITLE     An improved Tn7-lux reporter for broad host range, 
            chromosomally-integrated promoter fusions in Gram-negative bacteria.
  JOURNAL   J Microbiol Methods. 2015 Sep 1;118:75-77. doi: 
            10.1016/j.mimet.2015.08.016.
  PUBMED    26341612
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Microbiol
            Methods."; date: "2015-09-1"; volume: "118:75-77. doi"; pages: " 
            10.1016/j.mimet.2015.08.016"
COMMENT     Sequence Label: pAG4
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(128..175)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     terminator      complement(221..307)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      complement(410..504)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(532..548)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(533..549)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 agttgggcat acgggaagaa gtgatgcact ttgatatcga cccaagtacc gccacctaac
       61 aattcgttca agccgagatc aattccattg agtaagtttt taagcacatc agcttcaaaa
      121 gcgctctgaa gttcctatac tttctagaga ataggaactt cggaataggt acttcaagat
      181 ccccaattcg atcgtccggg ccgcaagctc ctagcggcgg atttgtccta ctcaggagag
      241 cgttcaccga caaacaacag ataaaacgaa aggcccagtc tttcgactga gcctttcgtt
      301 ttatttgatg cctcaagcta gagagtcatt accccaggcg tttaagggca ccaataactg
      361 ccttaaaaaa attacgcccc gccctgccac tcatcgcagt ctagcttgga ttctcaccaa
      421 taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca
      481 ttactggatc tatcaacagg agtccaagct cagctaatta agctagctta tcgataccgt
      541 cgacctcgaa ccccacgccc ctctttaata cgacgggcaa tttgcacttc agaaaatgaa
      601 gagtttgctt tagccataac aaaagtccag tatgcttttt cacagcataa ctggactgat
      661 ttcagtttac aactattctg tctagtttaa gactttattg tcatagttta gatctatttt
      721 gttcagttta agactttatt gtccgcccac acccgcttac gcagggcatc catttattac
      781 tcaaccgtaa ccgattt
//
