LOCUS       sequence-133331-        5445 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5445)
  AUTHORS   Bennett AM, Hausdorff SF, O'Reilly AM, Freeman RM, Neel BG
  TITLE     Multiple requirements for SHPTP2 in epidermal growth factor-mediated
            cell cycle progression.
  JOURNAL   Mol Cell Biol 1996 Mar;16(3):1189-202.
  PUBMED    8622663
REFERENCE   2  (bases 1 to 5445)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol"; date: "1996-03"; volume: "16(3)"; pages: "1189-202"
COMMENT     Sequence Label: pJ3 SHP2 WT
FEATURES             Location/Qualifiers
     source          1..5445
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(19..38)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     179..201
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(239..259)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        244..573
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      424..559
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          2683..2748
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2878..2898
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3323..3457
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3360..3379)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3414..3433
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        3488..3592
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3593..3661,3662..4453)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3811..3830)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4624..5212
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5113..5132
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5366..5383
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    join(5398..5445,1..93)
                     /label=bom
                     /note="basis of mobility region from pBR322"
ORIGIN
        1 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact
       61 ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac
      121 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
      181 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgtgg
      241 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
      301 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
      361 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
      421 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
      481 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
      541 ggaggctttt ttggaggcct aggcttttgc aaaagcttcg atcgtcgact ctagaggatc
      601 cccgggcgag ctcgaattcc gggcgggagg aacatgacat cgcggagatg gtttcaccca
      661 aatatcactg gtgtggaggc agaaaaccta ctgttgacaa gaggagttga tggcagtttt
      721 ttggcaaggc ctagtaaaag taaccctgga gacttcacac tttccgttag aagaaatgga
      781 gctgtcaccc acatcaagat tcagaacact ggtgattact atgacctgta tggaggggag
      841 aaatttgcca ctttggctga gttggtccag tattacatgg aacatcacgg gcaattaaaa
      901 gagaagaatg gagatgtcat tgagcttaaa tatcctctga actgtgcaga tcctacctct
      961 gaaaggtggt ttcatggaca tctctctggg aaagaagcag agaaattatt aactgaaaaa
     1021 ggaaaacatg gtagttttct tgtacgagag agccagagcc accctggaga ttttgttctt
     1081 tctgtgcgca ctggtgatga caaaggggag agcaatgacg gcaagtctaa agtgacccat
     1141 gttatgattc gctgtcagga actgaaatac gacgttggtg gaggagaacg gtttgattct
     1201 ttgacagatc ttgtggaaca ttataagaag aatcctatgg tggaaacatt gggtacagta
     1261 ctacaactca agcagcccct taacacgact cgtataaatg ctgctgaaat agaaagcaga
     1321 gttcgagaac taagcaaatt agctgagacc acagataaag tcaaacaagg cttttgggaa
     1381 gaatttgaga cactacaaca acaggagtgc aaacttctct acagccgaaa agagggtcaa
     1441 aggcaagaaa acaaaaacaa aaatagatat aaaaacatcc tgccctttga tcataccagg
     1501 gttgtcctac acgatggtga tcccaatgag cctgtttcag attacatcaa tgcaaatatc
     1561 atcatgcctg aatttgaaac caagtgcaac aattcaaagc ccaaaaagag ttacattgcc
     1621 acacaaggct gcctgcaaaa cacggtgaat gacttttggc ggatggtgtt ccaagaaaac
     1681 tcccgagtga ttgtcatgac aacgaaagaa gtggagagag gaaagagtaa atgtgtcaaa
     1741 tactggcctg atgagtatgc tctaaaagaa tatggcgtca tgcgtgttag gaacgtcaaa
     1801 gaaagcgccg ctcatgacta tacgctaaga gaacttaaac tttcaaaggt tggacaaggg
     1861 aatacggaga gaacggtctg gcaataccac tttcggacct ggccggacca cggcgtgccc
     1921 agcgaccctg ggggcgtgct ggacttcctg gaggaggtgc accataagca ggagagcatc
     1981 atggatgcag ggccggtcgt ggtgcactgc agtgctggaa ttggccggac agggacgttc
     2041 attgtgattg atattcttat tgacatcatc agagagaaag gtgttgactg cgatattgac
     2101 gttcccaaaa ccatccagat ggtgcggtct cagaggtcag ggatggtcca gacagaagca
     2161 cagtaccgat ttatctatat ggcggtccag cattatattg aaacactaca gcgcaggatt
     2221 gaagaagagc agaaaagcaa gaggaaaggg cacgaatata caaatattaa gtattctcta
     2281 gcggaccaga cgagtggaga tcagagccct ctcccgcctt gtactccaac gccaccctgt
     2341 gcagaaatga gagaagacag tgctagagtc tatgaaaacg tgggcctgat gcaacagcag
     2401 aaaagtttca gatgagaaaa cctgccaaaa cttcagcaca gaaatagatg tggactttca
     2461 ccctctccct aaaaagatca agaacagacg caagaaagtt tatgtgaaga cagaatttgg
     2521 atttggaagg cttgcaatgt ggttgactac cttttgataa gcaaaatttg aaaccattta
     2581 aagaccggaa ttcatcgatg gtaccagatc tttgtgaagg aaccttactt ctgtggtgtg
     2641 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
     2701 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
     2761 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
     2821 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
     2881 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
     2941 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
     3001 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
     3061 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
     3121 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
     3181 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
     3241 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
     3301 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     3361 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     3421 gtccaaactc atcaatgtat cttatcatgt ctggatctct gcaggtggca cttttcgggg
     3481 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     3541 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     3601 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     3661 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     3721 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     3781 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     3841 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     3901 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     3961 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     4021 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     4081 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     4141 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     4201 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     4261 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     4321 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     4381 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     4441 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     4501 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     4561 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     4621 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     4681 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     4741 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     4801 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     4861 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     4921 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     4981 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     5041 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     5101 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     5161 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     5221 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     5281 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     5341 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct
     5401 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccg
//
