LOCUS       sequence-133365-         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This E. coli DH10B strain harbors a shuttle vector plasmid that 
            expresses the Mixed Feedback Loop version of the UBER system with a 
            T7RNAP translation rate of 300 and a TetR translation rate of 469.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Kushwaha M, Salis HM
  TITLE     A portable expression resource for engineering cross-species genetic
            circuits and pathways.
  JOURNAL   Nat Commun. 2015 Jul 17;6:7832. doi: 10.1038/ncomms8832.
  PUBMED    26184393
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-07-17"; volume: "6:7832. doi"; pages: " 
            10.1038/ncomms8832"
COMMENT     Sequence Label: MK1282
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     184..203
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        184..202
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             291..311
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 catgatcata tggcatgcat gtgctctgta tgtatataaa actcttgttt tcttcttttc
       61 tctaaatatt ctttccttat acattaggtc ctttgtagca taaattacta tacttctata
      121 gacacgcaaa cacaaataca cacactaaat taataactag ttctatcatt gataggtata
      181 aattaatacg actcactata gggagaccta tcagtgatag aggatccaac cgagcccaat
      241 ataggactca gggtgccaaa aaaatgtcta ccaccatggg aattcaacct cctaaaaaga
      301 aacgtaaagt taatactatt aatattgcta aaaatgactt ctcagatatt gaattagcag
      361 ccattccatt taatacatta gcagatcact atggtgaacg tttagcacgt gaacagttag
      421 cattagaaca tgaatcatat gaaatgggtg aagcacgttt tcgtaagatg ttcgagcgtc
      481 agttaaaagc aggtgaagtt gcagataatg cagcagccaa acctttaatt actacattat
      541 tacctaaaat gattgctcgt attaacgatt ggtttgaaga ggttaaagca aagcgtggta
      601 aacgtcctac agcatttcag ttcttacaag aaatcaaacc tgaagcagtt gcatatatta
      661 ctattaaaac aacattagca tgtttaacat cagcagataa tacaacagtt caagcagttg
      721 catcagcaat tggtcgtgca attgaagatg aagcacgttt tggtcgtatt cgtgatttag
      781 aagccaaaca ttttaaa
//
