LOCUS       sequence-133500-         959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Certo MT, Ryu BY, Annis JE, Garibov M, Jarjour J, Rawlings DJ, 
            Scharenberg AM
  TITLE     Tracking genome engineering outcome at individual DNA breakpoints.
  JOURNAL   Nat Methods. 2011 Jul 10;8(8):671-6. doi: 10.1038/nmeth.1648.
  PUBMED    21743461
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-07-10- 10"; volume: "8"; 
            issue: "8"; pages: "671-6"
COMMENT     Sequence Label: pCVL SFFV-EF1s HA.NLS.Sce(opt)
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(751..771)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(781..807)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             complement(781..807)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      813..822
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aattttgtaa tccagaggtt gattgtcgaa tgcattacct gcaggtcact tcaggaaggt
       61 ctcgctgctg atggtgttgg gcagcttgta catcatctgg gggatcaggt agggcttgat
      121 caggttgtag aagatcaggt agctcatgct gtcgatgtag atgatgggct tgttcttgtt
      181 gatcttcacg tagcagttca gctggaactt gttcctcagg cccttcacca ggtactccac
      241 ctcctcgaag gtgaagctct gggtgttcag cacgatgctc ttgttggtgc tgttcttgtt
      301 gtagtcccac ttgccgccgt cgtccatgaa ccagtaggcc aggctcatgg gggtcaggta
      361 gttctccacc aggttgttgg ggatggtctt cttgttgttc acgatgaaca ggttggccag
      421 cttgttgaag gcctggtgct tgaaggtctg ggcgccccag gtgatgacca ggttgcccag
      481 gtggttcacc ctctccttct tgtggggggg gctcagcacc cactggtcgt acagcaggca
      541 cacgtggtcc atgtaggcct tgttcttcca ctcgaactgc atgcagtagg tcttgccctc
      601 gtccctgctc ctgatgtagg cgtcgcccag gatcaggccg atgccggcct cgaactgctc
      661 gatgttcagc tcgatcagct ggctcttgta ctccttcagc agcttgctgt tggggcccag
      721 gttcatgacc tggttcttct tgatgttctt caccttcctc tttttcttag ggggcccggg
      781 ggcgtagtcg ggcacgtcgt aggggtatac gcccatggtg gcggcagatc ctgtgttctg
      841 gcggcaaacc cgttgcgaaa aagaacgttc acggcgacta ctgcacttat atacggttct
      901 cccccaccct cgggaaaaag gcggagccag tacacgacat cactttccca gtttacccc
//
