LOCUS       sequence-133618-         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytosolic FRET, Excitation: 504 / 554, Emission: 517 /
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Davidson Lab
  TITLE     FRET Indicators - Live and Fixed Cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: mPA-GFP-tdTomato-FRET-10
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(129..380)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSHQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(171..192)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     520..539
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     779..800
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
       61 ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatct agagtcgcgg
      121 ccgctttact tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc
      181 agcaggacca tgtgatcgcg cttctcgttg gggtctttgc tcagcttgga ctggtggctc
      241 aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag
      301 tggtcggcga gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa gttggccttg
      361 atgccgttct tctgcttgtc ggccatgata tagacgttgt ggctgttgta gttgtactcc
      421 agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg
      481 ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc
      541 ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca tggcggactt gaagaagtcg
      601 tgctgcttca tgtggtcggg gtagcggctg aagcactgca cgccgtagct gaaggtggtc
      661 acgagggtgg gccagggcac gggcagcttg ccggtggtgc agatgaactt cagggtcagc
      721 ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg
      781 tcgccgtcca gctcgaccag gatgggcacc accccggtga acagctcctc gccc
//
