LOCUS       sequence-133625-         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 505 / 569, Emission: 516
            / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos4a-H2B-C-10
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             100..477
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="PEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKVL
                     KQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPG
                     ELAKHAVSEGTKAITKYTSAK"
     polyA_signal    607..728
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(644..663)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     698..717
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(872..893)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gattacaaca aggttaagct gtatgagcat gctgttgctc attctggatt gcctgacaat
       61 gccagacgat ccggactcag atctggaggc tccggaggcc cagagccagc gaagtctgct
      121 cccgccccga aaaagggctc caagaaggcg gtgactaagg cgcagaagaa aggcggcaag
      181 aagcgcaagc gcagccgcaa ggagagctat tccatctatg tgtacaaggt tctgaagcag
      241 gtccaccctg acaccggcat ttcgtccaag gccatgggca tcatgaattc gtttgtgaac
      301 gacattttcg agcgcatcgc aggtgaggct tcccgcctgg cgcattacaa caagcgctcg
      361 accatcacct ccagggagat ccagacggcc gtgcgcctgc tgctgcctgg ggagttggcc
      421 aagcacgccg tgtccgaggg tactaaggcc atcaccaagt acaccagcgc taagtaagga
      481 tccaccggat ctagataact gatcataatc agccatacca catttgtaga ggttttactt
      541 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
      601 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
      661 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
      721 gtatcttaac gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
      781 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
      841 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaa
//
