LOCUS       sequence-133738-        1104 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RNA-OUT A4 variant - antisense (UTR sequence from Mutalik et al., 
            Nat. Chem. Biol., 2012).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Watters KE, Abbott TR, Lucks JB
  TITLE     Simultaneous characterization of cellular RNA structure and function
            with in-cell SHAPE-Seq.
  JOURNAL   Nucleic Acids Res. 2015 Sep 8. pii: gkv879.
  PUBMED    26350218
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Sep 8. pii: gkv879."
COMMENT     Sequence Label: pJBL-RNAOUTA4
FEATURES             Location/Qualifiers
     source          1..1104
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(205..309)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     377..395
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(399..442)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(666..683)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(917..936)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
       61 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
      121 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
      181 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
      241 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
      301 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
      361 gacattaacc tataaaaata ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt
      421 agctttcgct aaggatgatt tctggaattc taaagatctt tgacagctag ctcagtccta
      481 ggtataatga attctcgcac atcttgttgt ctgattattg atttttggcg aaaccatttg
      541 atcatatgac aagatgtgta tgcctctacc tgcttcggcc gataaagccg acgataatac
      601 tcccaaagcc cgccgaaagg cgggcttttt tttggatcct tactcgagtc tagactgcag
      661 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      721 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      781 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
      841 cacaggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      901 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      961 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     1021 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     1081 ctgggctgtg tgcacgaacc cccc
//
