LOCUS       sequence-133771-         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Fusco D, Accornero N, Lavoie B, Shenoy SM, Blanchard JM, Singer RH, 
            Bertrand E
  TITLE     Single mRNA molecules demonstrate probabilistic movement in living 
            mammalian cells.
  JOURNAL   Curr Biol. 2003 Jan 21. 13(2):161-7.
  PUBMED    12546792
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol. 
            2003 Jan 21. 13(2):161-7."
COMMENT     Sequence Label: pMS2-YFP
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(80..100)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(101..811,812..814,815..817))
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(143..164)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     492..511
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     751..772
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      814..823
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atcaagggtc cccaaactca ccctgaagtt ctcagctcta gctagtcagc tgccggcgag
       61 ctactgcaga atcgatttat acctttctct tcttttttgg cttgtacagc tcgtccatgc
      121 cgagagtgat cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt
      181 tggggtcttt gctcagggcg gactggtagc tcaggtagtg gttgtcgggc agcagcacgg
      241 ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc gagctgcacg ctgccgtcct
      301 cgatgttgtg gcggatcttg aagttcacct tgatgccgtt cttctgcttg tcggccatga
      361 tatagacgtt gtggctgttg tagttgtact ccagcttgtg ccccaggatg ttgccgtcct
      421 ccttgaagtc gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca
      481 cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt
      541 agccttcggg catggcggac ttgaagaagt cgtgctgctt catgtggtcg gggtagcggg
      601 cgaagcactg caggccgtag ccgaaggtgg tcacgagggt gggccagggc acgggcagct
      661 tgccggtggt gcagatgaac ttcagggtca gcttgccgta ggtggcatcg ccctcgccct
      721 cgccggacac gctgaacttg tggccgttta cgtcgccgtc cagctcgacc aggatgggca
      781 ccaccccggt gaacagctcc tcgcccttgc tcaccatggt ggcgaccggt ggatccgcgt
      841 agatgccgga gtttgctgcg attgctga
//
