LOCUS       sequence-133833-        2136 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Streptavidin(II) N-terminal tag (5' TAAA/3' CATA fusion).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-Strep(II)
FEATURES             Location/Qualifiers
     source          1..2136
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             29..52
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     terminator      89..146
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(170..187)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(341..929)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(421..440)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1112..1206)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1227..1886)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1801..1820
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2048..2066
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2070..2113)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2116..2135
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
ORIGIN
        1 atctagagac gcataggtct cataaatgtg gagccacccg cagttcgaaa aaccgcataa
       61 gagacccatg ccgtctcatt agctgcagtc cggcaaaaaa gggcaaggtg tcaccaccct
      121 gccctttttc tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg
      181 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
      241 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
      301 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc
      361 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
      421 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
      481 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
      541 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
      601 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
      661 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
      721 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
      781 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
      841 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
      901 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
      961 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     1021 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     1081 agtaaacttg gtctgacagc tcgaggcttg gattctcacc aataaaaaac gcccggcggc
     1141 aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca
     1201 ggagtccaag cgagctcgat atcaaattac gccccgccct gccactcatc gcagtactgt
     1261 tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga
     1321 atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg
     1381 ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag
     1441 ggattggctg acacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt
     1501 tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg
     1561 tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg
     1621 tgaacactat cccatatcac cagctcaccg tctttcattg ccatacgaaa ttccggatga
     1681 gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc
     1741 tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga
     1801 gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg
     1861 gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac
     1921 tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg
     1981 tgcccgatca actcgagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     2041 ctataaaaat aggcgtatca cgaggcagaa tttcagataa aaaaaatcct tagctttcgc
     2101 taaggatgat ttctggaatt cgcggccgct tctaga
//
