LOCUS       sequence-133966-        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole 
            ribonucleotide mutase, Trp34>Phe/Trp165>Phe mutant (AaPurE1fwf).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Kappock
  TITLE     Single Trp variants of Acetobacter aceti PurE
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK698
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             652..663
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    682..698
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(706..712,713..729,730..735,736..750))
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    714..730
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(774..792)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg
       61 cggccgcaag cttgtcgacg gagctcgaat tctcatttat cttcggtaat gggagagttg
      121 gggacagagg ccgtctgcaa tgcgcgaaat gtttcaaggc gtgcggcaag ggccgggtta
      181 tacagtgcaa gaatggaggc ggccagcaag gcggcatttt tggcgccgga tgccccaata
      241 gccaaagtgc ccacaggcac gccgccgggc atctgcacaa tggaaagcag gctgtccatg
      301 cctttaagag cgcgagattc tactggaaca cccagcacgg gcaggcgtgt ccatgccgca
      361 cacatacccg gcagatgtgc agctccaccg gccccagcaa taatcacatt caggccgcgt
      421 tcggctgccg tacgcgcata atccgccaac cggtctggcg tacgatgtgc tgaaacaatg
      481 agggtttcat gcggaatttc cagctctgtc agcaatgcgt ctgcatggcg catggtttcg
      541 aagtctgact ggctgcccat gataatgccc acaacggggg cagaggctgc tttatcttct
      601 aaagcactgg aagcagaggg caggggggcg gtttcgctca tcatatgtta atttctcctc
      661 tttaatgaat tctgtgtgaa attgttatcc gctcacaatt gaatctatta taattgttat
      721 ccgctcacaa agcaaataaa ttttttatga tttctcgagg tgaagacgaa agggcctcgt
      781 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
      841 cacttttcgg ggagatctcg ggcagcgttg ggtcctggcc acgggtgcgc atgatcgtgc
      901 tcctgtcgtt gaggacccgg ctaggctggc ggggttgcct tactggttag cagaatgaat
      961 caccgatacg cgagcgaacg tgaagcgact gctgctgcaa aacgtctgcg acctgagcaa
     1021 caacatgaat ggtcttcggt ttccgtgttt cgtaaagtct ggaaa
//
