LOCUS       sequence-134060-        1115 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Treponema denticola 5-aminoimidazole ribonucleotide 
            carboxylase, Ser38>Asn/Phe79>Trp mutant (TdPurE2-S38N,F79W).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Kappock
  TITLE     Serine mutants of Treponema denticola PurE2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1115)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK689
FEATURES             Location/Qualifiers
     source          1..1115
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(33..50)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             611..633
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(664..683)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(665..683)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tcctttcggg ctttgttagc agccggatct cagtggtggt ggtggtggtg ctcgagtgcg
       61 gccgcaagct tgtcgacgga gctcgaattc gagctccgtc gacaagaaac tgaaacgctg
      121 aattcagcgt ttcagtttcg aatcgtcttc gataattttc tgcgcattgg attccatata
      181 ggatttgacc gaatctgcga tttctttatc gtacagtgaa aaaatacgag ccgccaggag
      241 tgccgcattt ttcggttcaa gaaccagtgc cggactaatc ccgctcggca tccgtaacga
      301 agaataaatg tcggcgccgg cgaagctatc gcttggcggt gggcaggcaa tggttgcacc
      361 tttgacccag ccatcaacaa agcctgataa agcgttacta cgcccagcga tcgtgatgta
      421 cagtttagga cggtccaaag cttcatattc tttcagcata gacactacat gctccgctgt
      481 tttatgcgcg ttaccgatgc gaatggcata ctcaatacca aaagttttca attcagacgc
      541 aattttctcg gcgtgaccca tatctgaact gctgcccata aggataatca ccagcggacg
      601 catatgtata tctccttctt aaagttaaac aaaattattt ctagagggaa accgttgtgg
      661 tctccctata gtgagtcgta ttaatttcgc gggatcgaga tctcgggcag cgttgggtcc
      721 tggccacggg tgcgcatgat cgtgctcctg tcgttgagga cccggctagg ctggcggggt
      781 tgccttactg gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc
      841 tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa
      901 agtctggaaa cgcggaagtc agcgccctgc accattatgt tccggatctg catcgcagga
      961 tgctgctggc taccctgtgg aacacctaca tctgtattaa cgaagcgctg gcattgaccc
     1021 tgagtgattt ttctctggtc ccgccgcatc cataccgcca gttgtttacc ctcacaacgt
     1081 tccagtaacc gggcatgttc atcatcagta acccg
//
