LOCUS       sequence-134130-         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains 600 bp homology arms to SS9 for one-step markerless genome 
            integration in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Bassalo MC, Garst AD, Halweg-Edwards AL, Grau WC, Domaille DW, 
            Mutalik VK, Arkin AP, Gill RT
  TITLE     Rapid and Efficient One-Step Metabolic Pathway Integration in E. 
            coli.
  JOURNAL   ACS Synth Biol. 2016 Apr 22.
  PUBMED    27072506
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Apr 22."
COMMENT     Sequence Label: pSS9
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(689..793)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ataatgaatt ccaactgagc gccggtcgct accattacca acttgtctgg tgtcaaaaat
       61 aataggccta ctagtcggcc gtacgggctt attatatcgc gttgattatt gatgctgttt
      121 ttagttttaa cggcaattaa tatatgtgtt attaattgaa tgaattttat cattcataat
      181 aagtatgtgt aggatcaagc tcaggttaaa tattcactca ggaagttatt actcaggaag
      241 caaagaggat tacagaatta tctcataaca agtgttaagg gatgttattt cccgattctc
      301 tgtggcataa taaacgagta gatgctcatt ccatctctta tgttcgcctt agtgcctcat
      361 aaactccgga atgacgcaga gccgtttacg gtgcttatcg tccactgaca gatgtcgctt
      421 atgcctcatc agacaccatg gacacaacgt tgagtgaagc acccacttgt tgtcatacag
      481 acctgtttta acgcctgctc cgtaataaga gcaggcgttt ttttatgtat caggaaggcc
      541 ccggaggtgc ttgcctccgg gtgagaagga actactgtgg cgggttattc tgcaacgtta
      601 acatcaaacc gtcgcgacgc atagctgcag cttcttccgg cttgtgcagt ctgtccagcg
      661 cgtcggcaag ccatgcgtaa tcgtaggcac tcttcctttt tcaatattat tgaagcattt
      721 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
      781 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
      841 tcatgacatt aacctataaa
//
