LOCUS       sequence-134132-         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SS9-targeting gRNA for co-transformation with pSS9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Bassalo MC, Garst AD, Halweg-Edwards AL, Grau WC, Domaille DW, 
            Mutalik VK, Arkin AP, Gill RT
  TITLE     Rapid and Efficient One-Step Metabolic Pathway Integration in E. 
            coli.
  JOURNAL   ACS Synth Biol. 2016 Apr 22.
  PUBMED    27072506
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Apr 22."
COMMENT     Sequence Label: SS9_RNA
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     81..99
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(103..146)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     promoter        164..198
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     misc_RNA        219..294
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             315..344
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             360..377
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(433..450)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      603..649
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(702..719)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
       61 tcatgacatt aacctataaa aataggcgta tcacgaggca gaatttcaga taaaaaaaat
      121 ccttagcttt cgctaaggat gatttctgga attctaaaga tctttgacag ctagctcagt
      181 cctaggtata atactagttc tggcgcagtt gatatgtagt tttagagcta gaaatagcaa
      241 gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt
      301 tgaagcttgg gcccgaacaa aaactcatct cagaagagga tctgaatagc gccgtcgacc
      361 atcatcatca tcatcattga gtttaaacgg tctccagctt ggctgttttg gcggatgaga
      421 gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga taaaacagaa
      481 tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact cagaaatgaa
      541 acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga actgccaggc
      601 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
      661 cggtgaactg gatccttact cgagtctaga ctgcaggctt cctcgctcac tgactcgctg
      721 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
      781 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
      841 aggaaccgta aaaaggccgc gttgctggcg ttt
//
