LOCUS       sequence-134134-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SS9-targeting gRNA for co-transformation with pSS9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Bassalo MC, Garst AD, Halweg-Edwards AL, Grau WC, Domaille DW, 
            Mutalik VK, Arkin AP, Gill RT
  TITLE     Rapid and Efficient One-Step Metabolic Pathway Integration in E. 
            coli.
  JOURNAL   ACS Synth Biol. 2016 Apr 22.
  PUBMED    27072506
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Apr 22."
COMMENT     Sequence Label: SS9_RNA
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     97..114
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     CDS             complement(170..187)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(203..232)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_RNA        complement(253..328)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(349..383)
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     terminator      401..444
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(448..466)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        534..638
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cggcgtttca tttctgagtt cggcatgggg tcaggtggga ccaccgcgct actgccgcca
       61 ggcaaattct gttttatcag accgcttctg cgttctgatt taatctgtat caggctgaaa
      121 atcttctctc atccgccaaa acagccaagc tggagaccgt ttaaactcaa tgatgatgat
      181 gatgatggtc gacggcgcta ttcagatcct cttctgagat gagtttttgt tcgggcccaa
      241 gcttcaaaaa aagcaccgac tcggtgccac tttttcaagt tgataacgga ctagccttat
      301 tttaacttgc tatttctagc tctaaaacta catatcaact gcgccagaac tagtattata
      361 cctaggactg agctagctgt caaagatctt tagaattcca gaaatcatcc ttagcgaaag
      421 ctaaggattt tttttatctg aaattctgcc tcgtgatacg cctattttta taggttaatg
      481 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
      541 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
      601 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
      661 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
      721 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
      781 atctcaacag cggtaagatc
//
